北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    Tet-pLKO-neo
Tet-pLKO-neoMap

Tet-pLKO-neo

Tet-pLKO-neo

Tet-pLKO-neo


 

编号

载体名称

北京华越洋VECT23118

Tet-pLKO-neo

 

Tet-pLKO-neo载体基本信息:

载体名称:

Tet-pLKO-neo

质粒类型:

慢病毒载体;RNAi载体;四环素诱导载体;Tet-On载体

高拷贝/低拷贝:

低拷贝

克隆方法:

限制性内切酶;多克隆位点

启动子:

--

载体大小:

10959 bp

5' 测序引物及序列:

5’   GGCAGGGATATTCACCATTATCGTTTCAGA 3’

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素

筛选标记:

Neomycin

克隆菌株:

Stbl3 感受态细胞 ,以维持质粒的稳定性。

宿主细胞(系):

哺乳动物细胞

备注:

其它筛选标记 : Tet-pLKO-puro

稳定性:

稳表达

组成型/诱导型:

诱导型

病毒/非病毒:

慢病毒

 

Tet-pLKO-neo载体质粒图谱和多克隆位点信息:

Tet-pLKO-neo载体序列:

ORIGIN

        1 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA

       61 AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT

      121 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA

      181 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT

      241 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA

      301 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC

      361 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT

      421 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT

      481 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT

      541 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC

      601 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG

      661 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC

      721 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA

      781 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG

      841 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT

      901 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT

      961 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA

     1021 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC

     1081 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC

     1141 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC

     1201 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG

     1261 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG

     1321 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA

     1381 AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG

     1441 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG

     1501 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA

     1561 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC

     1621 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT

     1681 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC

     1741 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA

     1801 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC

     1861 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG

     1921 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG

     1981 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC

     2041 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC

     2101 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC

     2161 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA

     2221 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG

     2281 CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC AACATGGTAA

     2341 CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC CGATTGGTGG

     2401 AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG ACATGGATTG

     2461 GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA GCTCGATACA

     2521 TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA

     2581 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC

     2641 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT

     2701 CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG AGCTCTCTCG

     2761 ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC GACTGGTGAG

     2821 TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG AGAGCGTCAG

     2881 TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC CAGGGGGAAA

     2941 GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC GATTCGCAGT

     3001 TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC AGCTACAACC

     3061 ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG CAACCCTCTA

     3121 TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA AGATAGAGGA

     3181 AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC AGACCTGGAG

     3241 GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA GTAAAAATTG

     3301 AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA GAAAAAAGAG

     3361 CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC ACTATGGGCG

     3421 CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA GTGCAGCAGC

     3481 AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC ACAGTCTGGG

     3541 GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG GATCAACAGC

     3601 TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG CCTTGGAATG

     3661 CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG ATGGAGTGGG

     3721 ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA TCGCAAAACC

     3781 AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT TTGTGGAATT

     3841 GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA GTAGGAGGCT

     3901 TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT AGGCAGGGAT

     3961 ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC AGGCCCGAAG

     4021 GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA GTGAACGGAT

     4081 CTCGACGGTA TCGATCACGA GACTAGCCTC GAGCGGCCGC AATATTTGCA TGTCGCTATG

     4141 TGTTCTGGGA AATCACCATA AACGTGAAAT CCCTATCAGT GATAGAGACT TATAAGTTCC

     4201 CTATCAGTGA TAGAGACACC GGTGTGTTGT AAATGAGCAC ACAAAATACA CATGCTAAAA

     4261 TATTATATTC TATGACCTTT ATAAAATCAA CCAAAATCTT CTTTTTAATA ACTTTAGTAT

     4321 CAATAATTAG AATTTTTATG TTCCTTTTTG CAAACTTTTA ATAAAAATGA GCAAAATAAA

     4381 AAAACGCTAG TTTTAGTAAC TCGCGTTGTT TTCTTCACCT TTAATAATAG CTACTCCACC

     4441 ACTTGTTCCT AAGCGGTCAG CTCCTGCTTC AATCATTTTT TGAGCATCTT CAAATGTTCT

     4501 AACTCCACCA GCTGCTTTAA CTAAAGCATT GTCTTTAACA ACTGACTTCA TTAGTTTAAC

     4561 ATCTTCAAAT GTTGCACCTG ATTTTGAAAA TCCTGTTGAT GTTTTAACAA ATTCTAATCC

     4621 AGCTTCAACA GCTATTTCAC AAGCTTTCAT GATTTCTTCT TTTGTTAATA AACAATTTTC

     4681 CATAATACAT TTAACAACAT GTGATCCAGC TGCTTTTTTT ACAGCTTTCA TGTCTTCTAA

     4741 AACTAATTCA TAATTTTTGT CTTTTAATGC ACCAATATTT AATACCATAT CAATTTCTGT

     4801 TGCACCATCT TTAATTGCTT CAGAAACTTC GAATGCTTTT GTAGCTGTTG TGCATGCACC

     4861 TAGAGGAAAA CCTACAACAT TTGTTATTCC TACATTTGTG CCTTTTAATA ATTCTTTACA

     4921 ATAGCTTGTT CAATATGAAT TAACACAAAC TGTTGCAAAA TCAAATTCAA TTGCTTCATC

     4981 ACATAATTGT TTAATTTCAG CTTTCGTAGC ATCTTGTTTT AATAATGTGT GATCTATATA

     5041 TTTGTTTAGT TTCATTTTTT CTCCTATATA TTCATTTTTA ATTTTAATTC TTTAATAATT

     5101 TCGTCTACTT TAACTTTAGC GTTTTGAACA GATTCACCAA CACCTATAAA ATAAATTTTT

     5161 AGTTTAGGTT CAGTTCCACT TGGGCGAACA GCAAATCATG ACTTATCTTC TAAATAAAAT

     5221 TTTAGTAAGT CTTGTCCTGG CATATTATAC ATTCCATCGA TGTAGTCTTC AACATTAACA

     5281 ACTTTAAGTC CAGCAATTTG AGTTAAGGGT GTTGCTCTCA ATGATTTCAT TAATGGTTCA

     5341 ATTTTTAATT TCTTTTCTTC TGGTTTAAAA TTCAAGTTTA AAGTGAAAGT GTAATATGCA

     5401 CCCATTTCTT TAAATAAATC TTCTAAATAG TCTACTAATG TTTTATTTTG TTTTTTATAA

     5461 AATCAAGCAG CCTCTGCTAT TAATATAGAA GCTTGTATTC CATCTTTATC TCTAGCTGAG

     5521 TCATCAATTA CATATCCATA ACTTTCTTCA TAAGCAAAAA CAAAATTTAA TCCGTTATCT

     5581 TCTTCTTTAG CAATTTCTCT ACCCATTCAT TTAAATCCAG TTAAAGTTTT TACAATATTA

     5641 ACTCCATATT TTTCATGAGC GATTCTATCA CCCAAATCAC TTGTTACAAA ACTTGAATAT

     5701 AGAGCCGGAT TTTTTGGAAT GCTATTTAAG CGTTTTAGAT TTGATAATTT TCAATCAATT

     5761 AAAATTGGTC CTGTTTGATT TCCATCTAAT CTTACAAAAT GACCATCATG TTTTATTGCC

     5821 ATTCCAAATC TGTCAGCATC TGGGTCATTC ATAATAATAA TATCTGCATC ATGTTTAATA

     5881 CCATATTCAA GCGGTATTTT TCATGCAGGA TCAAATTCTG GATTTGGATT TACAACATTT

     5941 TTAAATGTTT CATCTTCAAA TGCATGCTCT TCAACCTCAA TAACGTTATA TCCTGATTCA

     6001 CGTAATATTT TTGGGGTAAA TTTAGTTCCT GTTCCATTAA CTGCGCTAAA AATAATTTTT

     6061 AAATCTTTTT TAGCTTCTTG CTCTTTTTTG TAGAATTCTC GACCTCGAGA CAAATGGCAG

     6121 TATTCATCCA CAATTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA

     6181 TAGTAGACAT AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAA

     6241 TTCAAAATTT TCGGGTTTAT TACAGGGACA GCAGAGATCC ACTTTGGCCG CGGCTCGAGG

     6301 GGGTTGGGGT TGCGCCTTTT CCAAGGCAGC CCTGGGTTTG CGCAGGGACG CGGCTGCTCT

     6361 GGGCGTGGTT CCGGGAAACG CAGCGGCGCC GACCCTGGGT CTCGCACATT CTTCACGTCC

     6421 GTTCGCAGCG TCACCCGGAT CTTCGCCGCT ACCCTTGTGG GCCCCCCGGC GACGCTTCCT

     6481 GCTCCGCCCC TAAGTCGGGA AGGTTCCTTG CGGTTCGCGG CGTGCCGGAC GTGACAAACG

     6541 GAAGCCGCAC GTCTCACTAG TACCCTCGCA GACGGACAGC GCCAGGGAGC AATGGCAGCG

     6601 CGCCGACCGC GATGGGCTGT GGCCAATAGC GGCTGCTCAG CAGGGCGCGC CGAGAGCAGC

     6661 GGCCGGGAAG GGGCGGTGCG GGAGGCGGGG TGTGGGGCGG TAGTGTGGGC CCTGTTCCTG

     6721 CCCGCGCGGT GTTCCGCATT CTGCAAGCCT CCGGAGCGCA CGTCGGCAGT CGGCTCCCTC

     6781 GTTGACCGAA TCACCGACCT CTCTCCCCAG GGGGATCTGT GAGTTTGGGG ACCCTTGATT

     6841 GTTCTTTCTT TTTCGCTATT GTAAAATTCA TGTTATATGG AGGGGGCAAA GTTTTCAGGG

     6901 TGTTGTTTAG AATGGGAAGA TGTCCCTTGT ATCACCATGG ACCCTCATGA TAATTTTGTT

     6961 TCTTTCACTT TCTACTCTGT TGACAACCAT TGTCTCCTCT TATTTTCTTT TCATTTTCTG

     7021 TAACTTTTTC GTTAAACTTT AGCTTGCATT TGTAACGAAT TTTTAAATTC ACTTTTGTTT

     7081 ATTTGTCAGA TTGTAAGTAC TTTCTCTAAT CACTTTTTTT TCAAGGCAAT CAGGGTATAT

     7141 TATATTGTAC TTCAGCACAG TTTTAGAGAA CAATTGTTAT AATTAAATGA TAAGGTAGAA

     7201 TATTTCTGCA TATAAATTCT GGCTGGCGTG GAAATATTCT TATTGGTAGA AACAACTACA

     7261 TCCTGGTCAT CATCCTGCCT TTCTCTTTAT GGTTACAATG ATATACACTG TTTGAGATGA

     7321 GGATAAAATA CTCTGAGTCC AAACCGGGCC CCTCTGCTAA CCATGTTCAT GCCTTCTTCT

     7381 TTTTCCTACA GCTCCTGGGC AACGTGCTGG TTATTGTGCT GTCTCATCAT TTTGGCAAAG

     7441 AATTGTAATA CGACTCACTA TAGGGCGAAT TGATATGTCT AGATTAGATA AAAGTAAAGT

     7501 GATTAACAGC GCATTAGAGC TGCTTAATGA GGTCGGAATC GAAGGTTTAA CAACCCGTAA

     7561 ACTCGCCCAG AAGCTAGGTG TAGAGCAGCC TACATTGTAT TGGCATGTAA AAAATAAGCG

     7621 GGCTTTGCTC GACGCCTTAG CCATTGAGAT GTTAGATAGG CACCATACTC ACTTTTGCCC

     7681 TTTAGAAGGG GAAAGCTGGC AAGATTTTTT ACGTAATAAC GCTAAAAGTT TTAGATGTGC

     7741 TTTACTAAGT CATCGCGATG GAGCAAAAGT ACATTTAGGT ACACGGCCTA CAGAAAAACA

     7801 GTATGAAACT CTCGAAAATC AATTAGCCTT TTTATGCCAA CAAGGTTTTT CACTAGAGAA

     7861 TGCATTATAT GCACTCAGCG CTGTGGGGCA TTTTACTTTA GGTTGCGTAT TGGAAGATCA

     7921 AGAGCATCAA GTCGCTAAAG AAGAAAGGGA AACACCTACT ACTGATAGTA TGCCGCCATT

     7981 ATTACGACAA GCTATCGAAT TATTTGATCA CCAAGGTGCA GAGCCAGCCT TCTTATTCGG

     8041 CCTTGAATTG ATCATATGCG GATTAGAAAA ACAACTTAAA TGTGAAAGTG GGTCCGCGTA

     8101 CAGCGGCTCC CGGGAGTTCT AGGGATCTGC CCCTCTCCCT CCCCCCCCCC TAACGTTACT

     8161 GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT TTCCACCATA

     8221 TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT GACGAGCATT

     8281 CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT CGTGAAGGAA

     8341 GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG

     8401 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT ATAAGATACA

     8461 CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT GGAAAGAGTC

     8521 AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG ATGCCCAGAA GGTACCCCAT

     8581 TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA CATGTGTTTA GTCGAGGTTA

     8641 AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA AACACGATGA

     8701 TAAGGATCCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG

     8761 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC

     8821 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG

     8881 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC

     8941 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG

     9001 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT

     9061 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG

     9121 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT

     9181 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC

     9241 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG

     9301 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC

     9361 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT

     9421 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT

     9481 CGCCTTCTTG ACGAGTTCTT CTGAGGTACC TTTTTTAAGG GTGGGCGCGC CGACCCAGCT

     9541 TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG

     9601 GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCG CCCGCCCCAC

     9661 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGGTACC TTTAAGACCA

     9721 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA

     9781 GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG

     9841 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT

     9901 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT

     9961 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA

    10021 TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG

    10081 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA

    10141 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT

    10201 TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC

    10261 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG

    10321 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG

    10381 GGACGTACCC AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT

    10441 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC

    10501 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT

    10561 GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT

    10621 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC

    10681 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG

    10741 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA

    10801 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT

    10861 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT

    10921 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGAT

//

 

pLVX-ZsGreen1-N1其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

pLVX-IRES-mCherry

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-tdTomato-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLVX-Tet-On-Advanced

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Hyg

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-AcGFP1-C1

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-IRES-ZsGreen1

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-Hom-Mem1

pLVX-MetLuc     Control

pLVX-EF1α-AcGFP1-N1

pCDH-CMV-MCS-EF1-Puro

pLVX-Het-2

pLVX-IRES-Neo

pPRIME-TET-GFP-FF3

pSIH1-H1-CopGFP

pLentilox     3.7

pLOX-CW-CRE

pRSV-rev

pLVX-DD-AmCyan1     Reporter

pLL3.7

pLVX-Het-1

pMDLg-pRRE

pLVX-DD-tdTomato     Reporter

pLVX-PTuner

pLVX-PTuner-Green

pLVX-CherryPicker2

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Puro

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-rHom-Sec1

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-Het-Nuc1

pLVX-DD-tdTomato     Control

pLVX-TetOne

pLVX-DD-ZsGreen1     Reporter

pLVX-DD-AmCyan1     Control

pLVX-rHom-Nuc1

pCDH-CMV-MCS-EF1-Neo

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pCDF1-MCS2-EF1-Puro

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-ZsGreen1

pLVX-TRE3G-mCherry

pLVX-CherryPicker     Control

pLenti6/V5-GW/lacZ

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pLenti6.3-DsRed2-BveI     miR

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

psPAX2

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

pcDNA6.2-DsRed2-MCS1     miR

FUGW

pLenti6.3-EmGFP-BveI     miR

pcDNA6.2-EmGFP-BsaI     miR

pGIPZ

pLenti6.3-MCS

pLEX-MCS

pLenti6.3-BveI     miR

pSicoR

pLP1

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

pLOX-Ttag-iresTK

VSV-G

pLentG-KOSM

pCMV-dR8.91

pMDLg/pRRE

pFUGW

pCgpv

pLVX-TRE3G-Luc     Control

pLP2

pLVTHM

pSico

pLenti6.3-MCS-IRES2-DsRed2

pPACKH1-REV

pGensil-1

pcDNA6.2-EmGFP-NC-     I

pLKO.1-puro-GFP-siRNA

FUW-tetO-hOCT4

pSico     PGK Puro

FUW-tetO-hMYC

FUW

pLVX-shRNA2

FUW-M2rtTA

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLVX-AmCyan1-C1

pLKO.1-TRC     control

pPACKH1-GAG

pLVX-DsRed-Monomer-C1

pMD2.G

pLKO.1-TRC

pLVX-ZsGreen1-C1

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLVX-IRES-tdTomato

pLKO.1-hygro

FUW-tetO-hKLF4

pLVX-tdTomato-N1

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

pLVX-Tight-Puro

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-EF1α-mCherry-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-EF1α-IRES-Puro

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pLVX-Het-Mem1

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1     Control

pLP/VSVG

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pcDNA6.2-EmGFP-MCS1     miR

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pcDNA6.2-BsaI     miR

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pTRIPZ

pLVX-Hom-1

pLVX-shRNA1

LeGO-iC2

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI     miR

pLKO.3G

pLVX-CherryPicker1

pLVX-TRE3G

pLVX-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pSicoR     PGK Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1