pCDH-EF1-MCS-T2A-Puro

pCDH-EF1-MCS-T2A-Puro

编号	载体名称
北京华越洋VECT2311101	pCDH-EF1-MCS-T2A-Puro

pCDH-EF1-MCS-T2A-Puro载体基本信息：

载体名称:	pCDH-EF1-MCS-T2A-Puro
质粒类型:	慢病毒表达载体；cDNA克隆载体
克隆方法:	多克隆位点，限制性内切酶
启动子:	EF1α
载体大小:	7095 bp
5' 测序引物及序列:	5’-CTCCACGCTTTGCCTGACCCTGCTT-3’
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	嘌呤霉素
克隆菌株:	stbl3，在30度条件下进行质粒扩增
宿主细胞（系）:	大部分细胞类型（分裂与非分裂细胞）；初级分化细胞
备注:	pCDH-EF1-MCS-T2A-Puro慢病毒表达载体是基于HIV的慢病毒载体；用于cDNA表达和克隆；高效转染细胞，建立稳定细胞系；单一启动子，由EF1a 启动子驱动目的基因和与抗性基因转录为双顺反子；二者之间含一段T2A肽，能够在翻译后水平上自我剪切，保证目的基因与抗性基因翻译水平相同。慢病毒包装质粒为pPACKH1。
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒（HIV)

pCDH-EF1-MCS-T2A-Puro载体质粒图谱和多克隆位点信息：

pCDH-EF1-MCS-T2A-Puro载体简介

pCDH-EF1-MCS-T2A-Puro载体序列

pCDH-EF1-MCS-T2A-Puro其他相关慢病毒载体：

pLVX-DsRed-Monomer-N1	pLVX-PAmCherry-C1	pLVX-IRES-mCherry
Tet-pLKO-neo	pLVX-DsRed-Express2-C1	pLVX-tdTomato-C1
pLVX-mCherry-C1	pLVX-AcGFP1-N1	pLVX-Tet-On-Advanced
pLKO.1-puro	FUW-tetO-hSOX2	pLVX-IRES-Hyg
pLVX-IRES-Puro	pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro	pLVX-EF1α-AcGFP1-C1
pLVX-EF1α-DsRed-Monomer-C1	pLVX-MetLuc	pLVX-EF1α-IRES-ZsGreen1
pLVX-EF1α-mCherry-C1	pLVX-EF1α-IRES-mCherry	pLVX-Hom-Mem1
pLVX-MetLuc Control	pLVX-EF1α-AcGFP1-N1	pCDH-CMV-MCS-EF1-Puro
pLVX-Het-2	pLVX-IRES-Neo	pPRIME-TET-GFP-FF3
pSIH1-H1-CopGFP	pLentilox 3.7	pLOX-CW-CRE
pRSV-rev	pLVX-DD-AmCyan1 Reporter	pLL3.7
pLVX-Het-1	pMDLg-pRRE	pLVX-DD-tdTomato Reporter
pLVX-PTuner	pLVX-PTuner-Green	pLVX-CherryPicker2
pLVX-rHom-1	pLOX-CWBmi1	pLVX-TetOne-Puro
pLVX-TetOne-Luc	pCDH-EF1-MCS-T2A-Puro	pLVX-rHom-Sec1
pLVX-TetOne-Puro-Luc	pLVX-DD-AcGFP1-Actin	pLVX-Het-Nuc1
pLVX-DD-tdTomato Control	pLVX-TetOne	pLVX-DD-ZsGreen1 Reporter
pLVX-DD-AmCyan1 Control	pLVX-rHom-Nuc1	pCDH-CMV-MCS-EF1-Neo
pLenti6.3-MCS-IRES2-EGFP	pLVX-Hom-Nuc1	pCDF1-MCS2-EF1-Puro
pLenti6.3/V5-GW/EmGFP	pLVX-PTuner2	pLVX-TRE3G-ZsGreen1
pLVX-TRE3G-mCherry	pLVX-CherryPicker Control	pLenti6/V5-GW/lacZ
pCDH-EF1-MCS-T2A-copGFP	pCDH-CMV-MCS-EF1-Hygro	pLenti6.3-DsRed2-BveI miR
pCDH-MCS-T2A-Puro-MSCV	pCDH1-MCS2-EF1-copGFP	psPAX2
pCDH-CMV-MCS-EF1-RFP-T2A-Puro	pWPXL	pcDNA6.2-DsRed2-MCS1 miR
FUGW	pLenti6.3-EmGFP-BveI miR	pcDNA6.2-EmGFP-BsaI miR
pGIPZ	pLenti6.3-MCS	pLEX-MCS
pLenti6.3-BveI miR	pSicoR	pLP1
pcDNA6.3-EmGFP-NC- II	pLVX-TRE3G-IRES	pLOX-Ttag-iresTK
VSV-G	pLentG-KOSM	pCMV-dR8.91
pMDLg/pRRE	pFUGW	pCgpv
pLVX-TRE3G-Luc Control	pLP2	pLVTHM
pSico	pLenti6.3-MCS-IRES2-DsRed2	pPACKH1-REV
pGensil-1	pcDNA6.2-EmGFP-NC- I	pLKO.1-puro-GFP-siRNA
FUW-tetO-hOCT4	pSico PGK Puro	FUW-tetO-hMYC
FUW	pLVX-shRNA2	FUW-M2rtTA
pCDH-MSCV-MCS-EF1-copGFP	pLKO.1-GFP-shRNA	pLVX-AmCyan1-C1
pLKO.1-TRC control	pPACKH1-GAG	pLVX-DsRed-Monomer-C1
pMD2.G	pLKO.1-TRC	pLVX-ZsGreen1-C1
pLVX-AmCyan1-N1	FUW-tetO-hOKMS	pLVX-IRES-tdTomato
pLKO.1-hygro	FUW-tetO-hKLF4	pLVX-tdTomato-N1
pCMV-dR8.2-dvpr	pLVX-AcGFP1-C1	pLVX-Tight-Puro
Tet-pLKO-puro	pLVX-DsRed-Express2-N1	pLVX-EF1α-mCherry-N1
pLVX-mCherry-N1	pLVX-PAmCherry-N1	pLVX-EF1α-IRES-Puro
pLVX-IRES-ZsGreen1	pLVX-PTuner2-C	pLVX-Het-Mem1
pPRIME-TREX-GFP-FF3	pLVX-DD-ZsGreen1 Control	pLP/VSVG
pCDH-CMV-MCS-EF1-copGFP	pCDH-UbC-MCS-EF1-Hygro	pcDNA6.2-EmGFP-MCS1 miR
pLOX-TERT-iresTK	pCDH-CMV-MCS-EF1-RFP	pcDNA6.2-BsaI miR
pCDH-EF1-MCS-(PGK-Puro)	pCDF1-MCS2-EF1-copGFP	pTRIPZ
pLVX-Hom-1	pLVX-shRNA1	LeGO-iC2
pLVX-mCherry-Actin	pcDNA6.2-DsRed2-BsmBI miR	pLKO.3G
pLVX-CherryPicker1	pLVX-TRE3G	pLVX-Puro
pCDH-CMV-MCS-EF1-copGFP-T2A-Puro		pSicoR PGK Puro
pCMV-DsRed-Express2	pLVX-TRE3G-Hom1