北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pLKO.1-TRC control
1

pLKO.1-TRC control

pLKO.1-TRC control

pLKO.1-TRC control

 



编号

载体名称

北京华越洋VECT231144

pLKO.1-TRC control

 

pLKO.1-TRC control载体基本信息:

载体名称:

pLKO.1-TRC control

质粒类型:

慢病毒载体;RNAi载体

高拷贝/低拷贝:

低拷贝

克隆方法:

AgeIEcoRI

启动子:

U6

载体大小:

7050 bp

5' 测序引物及序列:

LKO.15' GACTATCATATGCTTACCGT

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素

筛选标记:

Puromycin

克隆菌株:

Stbl3

宿主细胞(系):

哺乳动物细胞

备注:

Insert a non-hairpin 18bp.
  This plasmid is recommended by The RNAi Consortium for use as a non-hairpin   control
  The 18bp between AgeI and EcoRI of this plasmid are CCGCAGGTATGCACGCGT.
  The link to the author's map shows the key features of this vector before the   18bp were cloned in.

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

慢病毒

 

pLKO.1-TRC control载体质粒图谱和多克隆位点信息:

pLKO.1-TRC control载体序列:

ORIGIN

    1 TTGGGGTTGC GCCTTTTCCA AGGCAGCCCT GGGTTTGCGC AGGGACGCGG CTGCTCTGGG

   61 CGTGGTTCCG GGAAACGCAG CGGCGCCGAC CCTGGGTCTC GCACATTCTT CACGTCCGTT

  121 CGCAGCGTCA CCCGGATCTT CGCCGCTACC CTTGTGGGCC CCCCGGCGAC GCTTCCTGCT

  181 CCGCCCCTAA GTCGGGAAGG TTCCTTGCGG TTCGCGGCGT GCCGGACGTG ACAAACGGAA

  241 GCCGCACGTC TCACTAGTAC CCTCGCAGAC GGACAGCGCC AGGGAGCAAT GGCAGCGCGC

  301 CGACCGCGAT GGGCTGTGGC CAATAGCGGC TGCTCAGCAG GGCGCGCCGA GAGCAGCGGC

  361 CGGGAAGGGG CGGTGCGGGA GGCGGGGTGT GGGGCGGTAG TGTGGGCCCT GTTCCTGCCC

  421 GCGCGGTGTT CCGCATTCTG CAAGCCTCCG GAGCGCACGT CGGCAGTCGG CTCCCTCGTT

  481 GACCGAATCA CCGACCTCTC TCCCCAGGGG GATCCACCGG AGCTTACCAT GACCGAGTAC

  541 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC

  601 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG

  661 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG

  721 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG

  781 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC

  841 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC

  901 CTGGCCACCG TCGGCGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG

  961 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG

 1021 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG

 1081 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGACG CCCGCCCCAC

 1141 GACCCGCAGC GCCCGACCGA AAGGAGCGCA CGACCCCATG CATCGGTACC TTTAAGACCA

 1201 ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA

 1261 GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG

 1321 TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT

 1381 CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT

 1441 AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA

 1501 TGTCATCTTA TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG

 1561 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA

 1621 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT

 1681 TATCATGTCT GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC

 1741 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG

 1801 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG

 1861 GGACGTACCC AATTCGCCCT ATAGTGAGTC GTATTACGCG CGCTCACTGG CCGTCGTTTT

 1921 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC

 1981 CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT

 2041 GCGCAGCCTG AATGGCGAAT GGGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT

 2101 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC

 2161 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG

 2221 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA

 2281 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT

 2341 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT

 2401 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA

 2461 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTTAACAAA ATATTAACGC TTACAATTTA

 2521 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT

 2581 TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA

 2641 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT

 2701 TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG

 2761 TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT

 2821 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG

 2881 GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG

 2941 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA

 3001 AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG

 3061 ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA

 3121 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC

 3181 ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT

 3241 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA

 3301 CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG

 3361 CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA

 3421 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG

 3481 ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT

 3541 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT

 3601 AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA

 3661 GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA

 3721 ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT

 3781 TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG

 3841 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA

 3901 ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA

 3961 AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG

 4021 CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA

 4081 AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA

 4141 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC

 4201 GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC

 4261 CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT

 4321 GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT

 4381 GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG

 4441 GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA

 4501 TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT

 4561 GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG

 4621 TTGTGTGGAA TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC

 4681 GCCAAGCGCG CAATTAACCC TCACTAAAGG GAACAAAAGC TGGAGCTGCA AGCTTAATGT

 4741 AGTCTTATGC AATACTCTTG TAGTCTTGCA ACATGGTAAC GATGAGTTAG CAACATGCCT

 4801 TACAAGGAGA GAAAAAGCAC CGTGCATGCC GATTGGTGGA AGTAAGGTGG TACGATCGTG

 4861 CCTTATTAGG AAGGCAACAG ACGGGTCTGA CATGGATTGG ACGAACCACT GAATTGCCGC

 4921 ATTGCAGAGA TATTGTATTT AAGTGCCTAG CTCGATACAT AAACGGGTCT CTCTGGTTAG

 4981 ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT

 5041 AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT

 5101 AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG

 5161 GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA

 5221 AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG

 5281 CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG

 5341 ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA

 5401 TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC

 5461 ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA

 5521 AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA

 5581 GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC

 5641 CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT

 5701 GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA

 5761 CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT

 5821 TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG

 5881 TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA

 5941 TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA

 6001 GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT

 6061 CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC

 6121 TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA

 6181 CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG

 6241 AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC

 6301 TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT

 6361 TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA

 6421 CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG

 6481 AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGATCACGAG

 6541 ACTAGCCTCG AGCGGCCGCC CCCTTCACCG AGGGCCTATT TCCCATGATT CCTTCATATT

 6601 TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA

 6661 AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT

 6721 TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT

 6781 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGG TCCGCAGGTA TGCACGCGTG

 6841 AATTCTCGAC CTCGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA

 6901 TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA

 6961 AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA

 7021 GAGATCCACT TTGGCCGCGG CTCGAGGGGG

//

pLKO.1-TRC control其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

pLVX-IRES-mCherry

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-tdTomato-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLVX-Tet-On-Advanced

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Hyg

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-AcGFP1-C1

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-IRES-ZsGreen1

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-Hom-Mem1

pLVX-MetLuc     Control

pLVX-EF1α-AcGFP1-N1

pCDH-CMV-MCS-EF1-Puro

pLVX-Het-2

pLVX-IRES-Neo

pPRIME-TET-GFP-FF3

pSIH1-H1-CopGFP

pLentilox     3.7

pLOX-CW-CRE

pRSV-rev

pLVX-DD-AmCyan1     Reporter

pLL3.7

pLVX-Het-1

pMDLg-pRRE

pLVX-DD-tdTomato     Reporter

pLVX-PTuner

pLVX-PTuner-Green

pLVX-CherryPicker2

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Puro

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-rHom-Sec1

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-Het-Nuc1

pLVX-DD-tdTomato     Control

pLVX-TetOne

pLVX-DD-ZsGreen1     Reporter

pLVX-DD-AmCyan1     Control

pLVX-rHom-Nuc1

pCDH-CMV-MCS-EF1-Neo

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pCDF1-MCS2-EF1-Puro

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-ZsGreen1

pLVX-TRE3G-mCherry

pLVX-CherryPicker     Control

pLenti6/V5-GW/lacZ

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pLenti6.3-DsRed2-BveI     miR

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

psPAX2

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

pcDNA6.2-DsRed2-MCS1     miR

FUGW

pLenti6.3-EmGFP-BveI     miR

pcDNA6.2-EmGFP-BsaI     miR

pGIPZ

pLenti6.3-MCS

pLEX-MCS

pLenti6.3-BveI     miR

pSicoR

pLP1

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

pLOX-Ttag-iresTK

VSV-G

pLentG-KOSM

pCMV-dR8.91

pMDLg/pRRE

pFUGW

pCgpv

pLVX-TRE3G-Luc     Control

pLP2

pLVTHM

pSico

pLenti6.3-MCS-IRES2-DsRed2

pPACKH1-REV

pGensil-1

pcDNA6.2-EmGFP-NC-     I

pLKO.1-puro-GFP-siRNA

FUW-tetO-hOCT4

pSico     PGK Puro

FUW-tetO-hMYC

FUW

pLVX-shRNA2

FUW-M2rtTA

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLVX-AmCyan1-C1

pLKO.1-TRC     control

pPACKH1-GAG

pLVX-DsRed-Monomer-C1

pMD2.G

pLKO.1-TRC

pLVX-ZsGreen1-C1

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLVX-IRES-tdTomato

pLKO.1-hygro

FUW-tetO-hKLF4

pLVX-tdTomato-N1

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

pLVX-Tight-Puro

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-EF1α-mCherry-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-EF1α-IRES-Puro

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pLVX-Het-Mem1

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1     Control

pLP/VSVG

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pcDNA6.2-EmGFP-MCS1     miR

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pcDNA6.2-BsaI     miR

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pTRIPZ

pLVX-Hom-1

pLVX-shRNA1

LeGO-iC2

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI     miR

pLKO.3G

pLVX-CherryPicker1

pLVX-TRE3G

pLVX-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pSicoR     PGK Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1