北京华越洋生物
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7*24h全国热线:
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首页    载体及质粒    pWPXL
1

pWPXL

pWPXL

pWPXL


 

编号

载体名称

北京华越洋VECT231211

pWPXL

 

pWPXL载体基本信息:

载体名称:

pWPXL

质粒类型:

哺乳动物细胞慢病毒表达载体

高拷贝/低拷贝:

高拷贝

启动子:

EF-1   alpha

克隆方法:

多克隆位点,限制性内切酶

载体大小:

10510   bp

5' 测序引物及序列:

EF-1a   Forward:   TCAAGCCTCAGACAGTGGTTC

3' 测序引物及序列:

EGFP-N: CGTCGCCGTCCAGCTCGACCAG

载体标签:

C-EGFP

载体抗性:

氨苄

备注:

pWPXL载体是用来进行稳定型转基因表达。pWPXL载体含有EF-1alpha启动子和内含子,这使得基因能够高效表达。载体3‘ LTR区的LoxP被克隆至目的细胞的5’ LTR反转录区。这 使得之前在Cre酶作用下插入的基因得以移除出去。载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组。

稳定性:

/

组成型:

--

病毒/非病毒:

慢病毒

 

pWPXL载体质粒图谱和多克隆位点信息:

 

pWPXL载体序列

ORIGIN

    1 TTGGAAGGGC TAATTCACTC CCAAAGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC

   61 ACACAAGGCT ACTTCCCTGA TTAGCAGAAC TACACACCAG GGCCAGGGGT CAGATATCCA

  121 CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC CAGATAAGGT AGAAGAGGCC

  181 AATAAAGGAG AGAACACCAG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG

  241 GAGAGAGAAG TGTTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CGTGGCCCGA

  301 GAGCTGCATC CGGAGTACTT CAAGAACTGC TGATATCGAG CTTGCTACAA GGGACTTTCC

  361 GCTGGGGACT TTCCAGGGAG GCGTGGCCTG GGCGGGACTG GGGAGTGGCG AGCCCTCAGA

  421 TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG

  481 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC

  541 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT

  601 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA

  661 GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG

  721 GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG

  781 AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG

  841 GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG

  901 GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC

  961 TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA

 1021 TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC

 1081 ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG

 1141 CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA

 1201 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA

 1261 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT

 1321 CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG

 1381 ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA

 1441 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC

 1501 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT

 1561 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT

 1621 TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT

 1681 ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA

 1741 ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT

 1801 AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT

 1861 TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC

 1921 AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG

 1981 AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGATGTCGAC GATAAGCTTT

 2041 GCAAAGATGG ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT

 2101 CTTGAAAGGA GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC

 2161 AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG

 2221 CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG

 2281 AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC

 2341 CAGAACACAG GTAAGTGCCG TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG

 2401 CCCTTGCGTG CCTTGAATTA CTTCCACTGG CTGCAGTACG TGATTCTTGA TCCCGAGCTT

 2461 CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC TTCGCCTCGT

 2521 GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG GTGGCACCTT

 2581 CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG ATGACCTGCT

 2641 GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT GCACACTGGT

 2701 ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG CACATGTTCG

 2761 GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC GGGGGTAGTC TCAAGCTGGC

 2821 CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG CCCCGCCCTG GGCGGCAAGG

 2881 CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG CCCTGCTGCA

 2941 GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC ACCCACACAA

 3001 AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA GTACCGGGCG

 3061 CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT AGGTTGGGGG

 3121 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA

 3181 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC

 3241 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGA

 3301 ATTTCGACAT TTAAATTTAA TTAATCTCGA CGGTATCGGT TAACTTTTAA AAGAAAAGGG

 3361 GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA

 3421 ACTAAAGAAT TACAAAAACA AATTACAAAA ATTCAAAATT TTCCGATCAC GAGACTAGCC

 3481 TCGAGGTTTA AACTACGGGA TCCAGGCCTA AGCTTACGCG TCCTAGCGCT ACCGGTCGCC

 3541 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG

 3601 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC

 3661 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC

 3721 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG

 3781 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC

 3841 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC

 3901 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG

 3961 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG

 4021 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC

 4081 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC

 4141 CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG

 4201 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG

 4261 TCCGGACTCA GATCTCGATA GCTAGTAGCT AGCTAGCTAG TCGAGCTCAA CTTCGAATTC

 4321 CCGGGACTAG TCATATGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG

 4381 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA

 4441 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT

 4501 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT

 4561 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC

 4621 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG

 4681 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC

 4741 GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG

 4801 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT

 4861 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC

 4921 CTCCCCGCAT CGGTACGTAT GGCCAGGTAC CTTTAAGACC AATGACTTAC AAGGCAGCTG

 4981 TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA AGGGCTAATT CACTCCCAAC

 5041 GAAGACAAGA TGGGATCAAT TCACCATGGG AATAACTTCG TATAGCATAC ATTATACGAA

 5101 GTTATGCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG

 5161 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT

 5221 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT

 5281 TAGTCAGTGT GGAAAATCTC TAGCAGCATC TAGAATTAAT TCCGTGTATT CTATAGTGTC

 5341 ACCTAAATCG TATGTGTATG ATACATAAGG TTATGTATTA ATTGTAGCCG CGTTCTAACG

 5401 ACAATATGTA CAAGCCTAAT TGTGTAGCAT CTGGCTTACT GAAGCAGACC CTATCATCTC

 5461 TCTCGTAAAC TGCCGTCAGA GTCGGTTTGG TTGGACGAAC CTTCTGAGTT TCTGGTAACG

 5521 CCGTCCCGCA CCCGGAAATG GTCAGCGAAC CAATCAGCAG GGTCATCGCT AGCCAGATCC

 5581 TCTACGCCGG ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT

 5641 ATATCGCCGA CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT

 5701 GTTTCGGCGT GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT

 5761 TGCATGCACC ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT

 5821 TCCTAATGCA GGAGTCGCAT AAGGGAGAGC GTCGAATGGT GCACTCTCAG TACAATCTGC

 5881 TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA

 5941 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC

 6001 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA

 6061 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT

 6121 TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG

 6181 TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT

 6241 ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT

 6301 GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA

 6361 CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC

 6421 GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC

 6481 CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG

 6541 GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA

 6601 TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC

 6661 GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT

 6721 GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG

 6781 CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT

 6841 TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC

 6901 TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT

 6961 CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC

 7021 ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC

 7081 TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT

 7141 TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG

 7201 ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC

 7261 AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA

 7321 CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG

 7381 GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA

 7441 GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA

 7501 CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG

 7561 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG

 7621 GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG

 7681 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG

 7741 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC

 7801 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA

 7861 AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG

 7921 TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT

 7981 GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA

 8041 GAGCGCCCAA TACGCAAACC GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGCTGT

 8101 GGAATGTGTG TCAGTTAGGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC

 8161 AAAGCATGCA TCTCAATTAG TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG

 8221 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC

 8281 CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA

 8341 TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT

 8401 GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT TGGACACAAG ACAGGCTTGC

 8461 GAGATATGTT TGAGAATACC ACTTTATCCC GCGTCAGGGA GAGGCAGTGC GTAAAAAGAC

 8521 GCGGACTCAT GTGAAATACT GGTTTTTAGT GCGCCAGATC TCTATAATCT CGCGCAACCT

 8581 ATTTTCCCCT CGAACACTTT TTAAGCCGTA GATAAACAGG CTGGGACACT TCACATGAGC

 8641 GAAAAATACA TCGTCACCTG GGACATGTTG CAGATCCATG CACGTAAACT CGCAAGCCGA

 8701 CTGATGCCTT CTGAACAATG GAAAGGCATT ATTGCCGTAA GCCGTGGCGG TCTGTACCGG

 8761 GTGCGTTACT GGCGCGTGAA CTGGGTATTC GTCATGTCGA TACCGTTTGT ATTTCCAGCT

 8821 ACGATCACGA CAACCAGCGC GAGCTTAAAG TGCTGAAACG CGCAGAAGGC GATGGCGAAG

 8881 GCTTCATCGT TATTGATGAC CTGGTGGATA CCGGTGGTAC TGCGGTTGCG ATTCGTGAAA

 8941 TGTATCCAAA AGCGCACTTT GTCACCATCT TCGCAAAACC GGCTGGTCGT CCGCTGGTTG

 9001 ATGACTATGT TGTTGATATC CCGCAAGATA CCTGGATTGA ACAGCCGTGG GATATGGGCG

 9061 TCGTATTCGT CCCGCCAATC TCCGGTCGCT AATCTTTTCA ACGCCTGGCA CTGCCGGGCG

 9121 TTGTTCTTTT TAACTTCAGG CGGGTTACAA TAGTTTCCAG TAAGTATTCT GGAGGCTGCA

 9181 TCCATGACAC AGGCAAACCT GAGCGAAACC CTGTTCAAAC CCCGCTTTAA ACATCCTGAA

 9241 ACCTCGACGC TAGTCCGCCG CTTTAATCAC GGCGCACAAC CGCCTGTGCA GTCGGCCCTT

 9301 GATGGTAAAA CCATCCCTCA CTGGTATCGC ATGATTAACC GTCTGATGTG GATCTGGCGC

 9361 GGCATTGACC CACGCGAAAT CCTCGACGTC CAGGCACGTA TTGTGATGAG CGATGCCGAA

 9421 CGTACCGACG ATGATTTATA CGATACGGTG ATTGGCTACC GTGGCGGCAA CTGGATTTAT

 9481 GAGTGGGCCC CGGATCTTTG TGAAGGAACC TTACTTCTGT GGTGTGACAT AATTGGACAA

 9541 ACTACCTACA GAGATTTAAA GCTCTAAGGT AAATATAAAA TTTTTAAGTG TATAATGTGT

 9601 TAAACTACTG ATTCTAATTG TTTGTGTATT TTAGATTCCA ACCTATGGAA CTGATGAATG

 9661 GGAGCAGTGG TGGAATGCCT TTAATGAGGA AAACCTGTTT TGCTCAGAAG AAATGCCATC

 9721 TAGTGATGAT GAGGCTACTG CTGACTCTCA ACATTCTACT CCTCCAAAAA AGAAGAGAAA

 9781 GGTAGAAGAC CCCAAGGACT TTCCTTCAGA ATTGCTAAGT TTTTTGAGTC ATGCTGTGTT

 9841 TAGTAATAGA ACTCTTGCTT GCTTTGCTAT TTACACCACA AAGGAAAAAG CTGCACTGCT

 9901 ATACAAGAAA ATTATGGAAA AATATTCTGT AACCTTTATA AGTAGGCATA ACAGTTATAA

 9961 TCATAACATA CTGTTTTTTC TTACTCCACA CAGGCATAGA GTGTCTGCTA TTAATAACTA

10021 TGCTCAAAAA TTGTGTACCT TTAGCTTTTT AATTTGTAAA GGGGTTAATA AGGAATATTT

10081 GATGTATAGT GCCTTGACTA GAGATCATAA TCAGCCATAC CACATTTGTA GAGGTTTTAC

10141 TTGCTTTAAA AAACCTCCCA CACCTCCCCC TGAACCTGAA ACATAAAATG AATGCAATTG

10201 TTGTTGTTAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA

10261 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA

10321 ATGTATCTTA TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC

10381 TTCCCACCCC ATACCCTATT ACCACTGCCA ATTACCTAGT GGTTTCATTT ACTCTAAACC

10441 TGTGATTCCT CTGAATTATT TTCATTTTAA AGAAATTGTA TTTGTTAAAT ATGTACTACA

10501 AACTTAGTAG

//

 

pWPXL其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

pLVX-IRES-mCherry

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-tdTomato-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLVX-Tet-On-Advanced

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Hyg

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-AcGFP1-C1

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-IRES-ZsGreen1

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-Hom-Mem1

pLVX-MetLuc     Control

pLVX-EF1α-AcGFP1-N1

pCDH-CMV-MCS-EF1-Puro

pLVX-Het-2

pLVX-IRES-Neo

pPRIME-TET-GFP-FF3

pSIH1-H1-CopGFP

pLentilox     3.7

pLOX-CW-CRE

pRSV-rev

pLVX-DD-AmCyan1     Reporter

pLL3.7

pLVX-Het-1

pMDLg-pRRE

pLVX-DD-tdTomato     Reporter

pLVX-PTuner

pLVX-PTuner-Green

pLVX-CherryPicker2

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Puro

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-rHom-Sec1

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-Het-Nuc1

pLVX-DD-tdTomato     Control

pLVX-TetOne

pLVX-DD-ZsGreen1     Reporter

pLVX-DD-AmCyan1     Control

pLVX-rHom-Nuc1

pCDH-CMV-MCS-EF1-Neo

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pCDF1-MCS2-EF1-Puro

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-ZsGreen1

pLVX-TRE3G-mCherry

pLVX-CherryPicker     Control

pLenti6/V5-GW/lacZ

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pLenti6.3-DsRed2-BveI     miR

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

psPAX2

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

pcDNA6.2-DsRed2-MCS1     miR

FUGW

pLenti6.3-EmGFP-BveI     miR

pcDNA6.2-EmGFP-BsaI     miR

pGIPZ

pLenti6.3-MCS

pLEX-MCS

pLenti6.3-BveI     miR

pSicoR

pLP1

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

pLOX-Ttag-iresTK

VSV-G

pLentG-KOSM

pCMV-dR8.91

pMDLg/pRRE

pFUGW

pCgpv

pLVX-TRE3G-Luc     Control

pLP2

pLVTHM

pSico

pLenti6.3-MCS-IRES2-DsRed2

pPACKH1-REV

pGensil-1

pcDNA6.2-EmGFP-NC-     I

pLKO.1-puro-GFP-siRNA

FUW-tetO-hOCT4

pSico     PGK Puro

FUW-tetO-hMYC

FUW

pLVX-shRNA2

FUW-M2rtTA

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLVX-AmCyan1-C1

pLKO.1-TRC     control

pPACKH1-GAG

pLVX-DsRed-Monomer-C1

pMD2.G

pLKO.1-TRC

pLVX-ZsGreen1-C1

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLVX-IRES-tdTomato

pLKO.1-hygro

FUW-tetO-hKLF4

pLVX-tdTomato-N1

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

pLVX-Tight-Puro

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-EF1α-mCherry-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-EF1α-IRES-Puro

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pLVX-Het-Mem1

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1     Control

pLP/VSVG

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pcDNA6.2-EmGFP-MCS1     miR

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pcDNA6.2-BsaI     miR

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pTRIPZ

pLVX-Hom-1

pLVX-shRNA1

LeGO-iC2

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI     miR

pLKO.3G

pLVX-CherryPicker1

pLVX-TRE3G

pLVX-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pSicoR     PGK Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1