pLKO.1-puro-GFP-siRNA
pLKO.1-puro-GFP-siRNA
编号 | 载体名称 |
北京华越洋VECT231285 | pLKO.1-puro-GFP-siRNA |
pLKO.1-puro-GFP-siRNA载体基本信息：
载体名称: | pLKO.1-puro-GFP-siRNA |
质粒类型: | 慢病毒载体；RNAi载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | AgeI，EcoRI |
启动子: | U6 |
载体大小: | 7091 bp |
5' 测序引物及序列: | LKO.15' ：GACTATCATATGCTTACCGT |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | Puromycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 哺乳动物细胞 |
备注: |
siRNA directed against GFP: 5'-GCAAGCTGACCCTGAAGTTCA-3' |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 慢病毒 |
pLKO.1-puro-GFP-siRNA载体质粒图谱和多克隆位点信息：
pLKO.1-puro-GFP-siRNA载体序列：
ORIGIN
1 TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA
61 AATATTAACG CTTACAATTT AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT
121 TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA
181 ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT
241 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA
301 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC
361 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT
421 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT
481 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT
541 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC
601 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG
661 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC
721 ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA
781 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG
841 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT
901 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT
961 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA
1021 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC
1081 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC
1141 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC
1201 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG
1261 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG
1321 GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA
1381 AATACTGTTC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG
1441 CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG
1501 TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA
1561 ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC
1621 CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT
1681 CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC
1741 TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA
1801 TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
1861 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
1921 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
1981 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
2041 GCGCGTTGGC CGATTCATTA ATGCAGCTGG CACGACAGGT TTCCCGACTG GAAAGCGGGC
2101 AGTGAGCGCA ACGCAATTAA TGTGAGTTAG CTCACTCATT AGGCACCCCA GGCTTTACAC
2161 TTTATGCTTC CGGCTCGTAT GTTGTGTGGA ATTGTGAGCG GATAACAATT TCACACAGGA
2221 AACAGCTATG ACCATGATTA CGCCAAGCGC GCAATTAACC CTCACTAAAG GGAACAAAAG
2281 CTGGAGCTGC AAGCTTAATG TAGTCTTATG CAATACTCTT GTAGTCTTGC AACATGGTAA
2341 CGATGAGTTA GCAACATGCC TTACAAGGAG AGAAAAAGCA CCGTGCATGC CGATTGGTGG
2401 AAGTAAGGTG GTACGATCGT GCCTTATTAG GAAGGCAACA GACGGGTCTG ACATGGATTG
2461 GACGAACCAC TGAATTGCCG CATTGCAGAG ATATTGTATT TAAGTGCCTA GCTCGATACA
2521 TAAACGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA
2581 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC
2641 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT
2701 CTAGCAGTGG CGCCCGAACA GGGACTTGAA AGCGAAAGGG AAACCAGAGG AGCTCTCTCG
2761 ACGCAGGACT CGGCTTGCTG AAGCGCGCAC GGCAAGAGGC GAGGGGCGGC GACTGGTGAG
2821 TACGCCAAAA ATTTTGACTA GCGGAGGCTA GAAGGAGAGA GATGGGTGCG AGAGCGTCAG
2881 TATTAAGCGG GGGAGAATTA GATCGCGATG GGAAAAAATT CGGTTAAGGC CAGGGGGAAA
2941 GAAAAAATAT AAATTAAAAC ATATAGTATG GGCAAGCAGG GAGCTAGAAC GATTCGCAGT
3001 TAATCCTGGC CTGTTAGAAA CATCAGAAGG CTGTAGACAA ATACTGGGAC AGCTACAACC
3061 ATCCCTTCAG ACAGGATCAG AAGAACTTAG ATCATTATAT AATACAGTAG CAACCCTCTA
3121 TTGTGTGCAT CAAAGGATAG AGATAAAAGA CACCAAGGAA GCTTTAGACA AGATAGAGGA
3181 AGAGCAAAAC AAAAGTAAGA CCACCGCACA GCAAGCGGCC GCTGATCTTC AGACCTGGAG
3241 GAGGAGATAT GAGGGACAAT TGGAGAAGTG AATTATATAA ATATAAAGTA GTAAAAATTG
3301 AACCATTAGG AGTAGCACCC ACCAAGGCAA AGAGAAGAGT GGTGCAGAGA GAAAAAAGAG
3361 CAGTGGGAAT AGGAGCTTTG TTCCTTGGGT TCTTGGGAGC AGCAGGAAGC ACTATGGGCG
3421 CAGCGTCAAT GACGCTGACG GTACAGGCCA GACAATTATT GTCTGGTATA GTGCAGCAGC
3481 AGAACAATTT GCTGAGGGCT ATTGAGGCGC AACAGCATCT GTTGCAACTC ACAGTCTGGG
3541 GCATCAAGCA GCTCCAGGCA AGAATCCTGG CTGTGGAAAG ATACCTAAAG GATCAACAGC
3601 TCCTGGGGAT TTGGGGTTGC TCTGGAAAAC TCATTTGCAC CACTGCTGTG CCTTGGAATG
3661 CTAGTTGGAG TAATAAATCT CTGGAACAGA TTTGGAATCA CACGACCTGG ATGGAGTGGG
3721 ACAGAGAAAT TAACAATTAC ACAAGCTTAA TACACTCCTT AATTGAAGAA TCGCAAAACC
3781 AGCAAGAAAA GAATGAACAA GAATTATTGG AATTAGATAA ATGGGCAAGT TTGTGGAATT
3841 GGTTTAACAT AACAAATTGG CTGTGGTATA TAAAATTATT CATAATGATA GTAGGAGGCT
3901 TGGTAGGTTT AAGAATAGTT TTTGCTGTAC TTTCTATAGT GAATAGAGTT AGGCAGGGAT
3961 ATTCACCATT ATCGTTTCAG ACCCACCTCC CAACCCCGAG GGGACCCGAC AGGCCCGAAG
4021 GAATAGAAGA AGAAGGTGGA GAGAGAGACA GAGACAGATC CATTCGATTA GTGAACGGAT
4081 CTCGACGGTA TCGATCACGA GACTAGCCTC GAGCGGCCGC CCCCTTCACC GAGGGCCTAT
4141 TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG ATAATTGGAA
4201 TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA AAGTAATAAT
4261 TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT ATGCTTACCG
4321 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACACCG
4381 GGCCCGCAAG CTGACCCTGA AGTTCATTCA AGAGATGAAC TTCAGGGTCA GCTTGCTTTT
4441 TGAATTCTCG ACCTCGAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG
4501 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC
4561 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG
4621 CAGAGATCCA CTTTGGCCGC GGCTCGAGGG GGTTGGGGTT GCGCCTTTTC CAAGGCAGCC
4681 CTGGGTTTGC GCAGGGACGC GGCTGCTCTG GGCGTGGTTC CGGGAAACGC AGCGGCGCCG
4741 ACCCTGGGTC TCGCACATTC TTCACGTCCG TTCGCAGCGT CACCCGGATC TTCGCCGCTA
4801 CCCTTGTGGG CCCCCCGGCG ACGCTTCCTG CTCCGCCCCT AAGTCGGGAA GGTTCCTTGC
4861 GGTTCGCGGC GTGCCGGACG TGACAAACGG AAGCCGCACG TCTCACTAGT ACCCTCGCAG
4921 ACGGACAGCG CCAGGGAGCA ATGGCAGCGC GCCGACCGCG ATGGGCTGTG GCCAATAGCG
4981 GCTGCTCAGC AGGGCGCGCC GAGAGCAGCG GCCGGGAAGG GGCGGTGCGG GAGGCGGGGT
5041 GTGGGGCGGT AGTGTGGGCC CTGTTCCTGC CCGCGCGGTG TTCCGCATTC TGCAAGCCTC
5101 CGGAGCGCAC GTCGGCAGTC GGCTCCCTCG TTGACCGAAT CACCGACCTC TCTCCCCAGG
5161 GGGATCCACC GGAGCTTACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG
5221 ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC
5281 GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC
5341 TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG
5401 CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC
5461 GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG
5521 CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC
5581 ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG
5641 CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC
5701 TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA
5761 CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG
5821 CACGACCCCA TGCATCGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA
5881 GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG
5941 ATCTGCTTTT TGCTTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
6001 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
6061 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
6121 CAGTGTGGAA AATCTCTAGC AGTAGTAGTT CATGTCATCT TATTATTCAG TATTTATAAC
6181 TTGCAAAGAA ATGAATATCA GAGAGTGAGA GGAACTTGTT TATTGCAGCT TATAATGGTT
6241 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
6301 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGGCTCTAG CTATCCCGCC
6361 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG
6421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA
6481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGGACGTAC CCAATTCGCC CTATAGTGAG
6541 TCGTATTACG CGCGCTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC
6601 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA
6661 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGGACGCG
6721 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
6781 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
6841 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
6901 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
6961 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
7021 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
7081 ATTTTGCCGA T//
pLKO.1-puro-GFP-siRNA其他相关慢病毒载体：
