pROKII
pROKII
编号 | 载体名称 |
北京华越洋VECT75586 | pROKII |
pROKII载体基本信息：
启动子: | CaMV 35S |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，蛋白过表达载体 |
质粒大小: | 12880bp |
原核抗性: | Kan |
筛选标记: | Neo |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
pROKII载体图谱：
pROKII载体简介：
An intron sequence of Poptr;CYCD1;1 from Populus trichocarpa was ligated into binary vector,pROKⅡ, to obtain the construct for gene silencing in plants. The resulting pRNAi was used to silence the exogenous fusion gene, GUS:GFP, in transgenic tobacco transformed with pCAMBIA-1303 vector. The specific sequence from GUS and GFP genes was cloned into pRNAi, respectively, and both of them reduced the expression of GUS:GFP gene. The results implied that the present pRNAi could be used to silence some gene of interest.
pROKII载体序列：
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
5821 ggatccccgg gtaccgagct cgaatttccc cgatcgttca aacatttggc aataaagttt
5881 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
5941 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
6001 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
6061 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcactggccg
6121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
6181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
6241 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6361 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta
6541 taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa
6601 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
6661 tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg
6721 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
6781 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
6841 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
6901 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
6961 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc
7021 cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct
7081 gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag
7141 ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc
7201 tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg
7261 ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag
7321 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca
7381 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc
7441 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg
7501 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc
7561 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc
7621 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag
7681 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac
7741 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag
7801 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat
7861 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg
7921 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc
7981 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac
8041 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac
8101 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg
8161 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc
8221 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa
8281 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc
8341 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
8401 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
8461 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
8521 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
8581 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
8641 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
8701 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
8761 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
8821 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
8881 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
8941 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
9001 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
9061 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
9121 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
9181 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
9241 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg
9301 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
9361 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
9421 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
9481 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
9541 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
9601 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
9661 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
9721 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
9781 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata
9841 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
9901 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
9961 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
10021 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
10081 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
10141 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
10201 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
10261 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
10321 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
10381 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
10441 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
10501 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
10561 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
10621 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
10681 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
10741 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg
10801 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct
10861 tctgtgtcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa
10921 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag
10981 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat
11041 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt
11101 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca
11161 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct
11221 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac
11281 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat
11341 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca
11401 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac
11461 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg
11521 ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga
11581 atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat
11641 gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat
11701 ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata
11761 tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa
11821 tatggcgctt catagagtaa ttctgtaaag gtccaattct cgtttcatac ctcggtataa
11881 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc
11941 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc
12001 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca ccaggccgcc gccctcactg
12061 cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc
12121 gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact
12181 gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg
12241 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg
12301 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg
12361 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca
12421 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct
12481 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct atctgtcagt agtcgcgccc
12541 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
12601 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
12661 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
12721 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
12781 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
12841 tagacggccg ccagcccagc ggcgagggca accagcccgg
//
pROKII载体其他相关的植物过表达载体：
