pGWB504
pGWB504
编号 | 载体名称 |
北京华越洋VECT75591 | pGWB504 |
pGWB504载体基本信息：
启动子: | NOS,Lac |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，gateway载体 |
质粒大小: | 11872bp |
质粒标签: | ccdB |
原核抗性: | Spe |
筛选标记: | HygR |
克隆菌株: | DB3.1 |
培养条件: | 37℃，有氧 LB |
表达宿主: | 植物细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | M13R:CAGGAAACAGCTATGACC |
pGWB504载体图谱：
pGWB504载体简介：
1. All ImpGWB vectors confer resistance to spectinomycin in E. coli and Agrobacterium tumefaciens. 2. ImpGWB vectors confer resistance to kanamycin (pGWB4xx) or hygromycin (pGWB5xx) in plants.
pGWB504载体序列：
ORIGIN
1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
301 aagcttgcat gcctgcaggc tctaatcaca agtttgtaca aaaaagctga acgagaaacg
361 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
421 tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc gcattaggca
481 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
541 cggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
601 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
661 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
721 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
781 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
841 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
901 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
961 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
1021 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
1081 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
1141 aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
1201 agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca
1261 gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt
1321 atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag tgacagttga
1381 cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc
1441 acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag
1501 gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac
1561 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
1621 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
1681 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
1741 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
1801 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
1861 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
1921 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
1981 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
2041 tggtgatcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
2101 agctggacgg cgacgtgaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
2161 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
2221 ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc
2281 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
2341 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
2401 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
2461 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
2521 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
2581 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
2641 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
2701 acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg gacgagctgt
2761 acaagtaaag cggcccgagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
2821 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
2881 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
2941 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
3001 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg
3061 aaccaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
3121 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
3181 ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat taattcccga tctagtaaca
3241 tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg
3301 cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca
3361 tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca
3421 tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat
3481 cggggaaatt cgggggatcg atcccgcgaa ctgtggacga gaactgtgcc accaagcgta
3541 aggccgttct ctcgcatttg ccttgctagg ctcgcgcgag ttgctggctg aggcgttctc
3601 gaaatcagct cttgttcggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
3661 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
3721 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
3781 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
3841 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
3901 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
3961 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
4021 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
4081 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
4141 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
4201 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
4261 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
4321 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
4381 gggagatgca ataggtcagg ctctcgctga atgccccaat gtcaagcact tccggaatcg
4441 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
4501 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
4561 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
4621 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ttattgcccc ccgggatcag
4681 atctggattg agagtgaata tgagactcta attggatacc gaggggaatt tatggaacgt
4741 cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag gcgacttttg
4801 aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag aaacccgcgg
4861 ctgagtggct ccttcaacgt tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg
4921 cgtcatcggc gggggtcata acgtgactcc cttaattctc cgctcatgat cttgatcccc
4981 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
5041 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
5101 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
5161 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
5221 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag tgcttgcggc
5281 agcgtgaagc tgatccgtcg agattaatag aaataaattc agcctaattc ggcgttaatt
5341 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
5401 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
5461 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
5521 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
5581 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
5641 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
5701 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
5761 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
5821 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
5881 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
5941 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
6001 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
6061 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
6121 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
6181 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
6241 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
6301 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
6361 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
6421 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
6481 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
6541 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
6601 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
6661 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
6721 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
6781 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
6841 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
6901 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6961 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
7021 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
7081 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
7141 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
7201 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
7261 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
7321 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
7381 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
7441 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
7501 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
7561 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
7621 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
7681 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
7741 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
7801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
7861 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
7921 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
7981 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
8041 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
8101 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
8161 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
8221 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
8281 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
8341 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
8401 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
8461 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
8521 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
8581 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
8641 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
8701 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
8761 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
8821 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
8881 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
8941 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
9001 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
9061 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
9121 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
9181 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
9241 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
9301 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
9361 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
9421 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
9481 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
9541 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
9601 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
9661 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
9721 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
9781 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
9841 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
9901 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
9961 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
10021 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
10081 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
10141 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
10201 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
10261 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
10321 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
10381 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
10441 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
10501 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
10561 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
10621 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
10681 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
10741 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
10801 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
10861 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
10921 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
10981 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
11041 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
11101 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
11161 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
11221 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
11281 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
11341 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
11401 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
11461 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
11521 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
11581 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
11641 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
11701 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
11761 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
11821 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac ct
//
pGWB504载体其他相关的GateWay载体：
