pBI221-EGFP
pBI221-EGFP
编号 | 载体名称 |
北京华越洋VECT75583 | pBI221-EGFP |
pBI221-EGFP载体基本信息：
启动子: | CaMV 35S ，Lac |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，蛋白过表达载体 |
质粒大小: | 4530bp |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
5'测序引物: | 35S:GACGCACAATCCCACTATCC |
3'测序引物: | 根据序列设计引物 |
pBI221-EGFP载体图谱：
pBI221-EGFP载体简介：
Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
pBI221-EGFP载体序列：
ORIGIN
1 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
61 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
121 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
181 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
241 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
301 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
361 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
421 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
481 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
541 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
601 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
661 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
721 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
781 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
841 ttcatttgga gagaacacgg gggactctag aggatccgaa ttcgagctcc gtcgacaagc
901 ttatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
961 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
1021 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
1081 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
1141 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
1201 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
1261 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
1321 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
1381 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
1441 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
1501 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
1561 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagt
1621 aaagcggccc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
1681 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
1741 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
1801 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
1861 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa
1921 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
1981 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
2041 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
2101 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
2161 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
2221 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
2281 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
2341 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
2401 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
2461 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
2521 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
2581 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
2641 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
2701 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
2761 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
2821 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
2881 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
2941 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
3001 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
3061 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
3121 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
3181 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
3241 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
3301 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
3361 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
3421 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
3481 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
3541 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
3601 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
3661 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
3721 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
3781 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
3841 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
3901 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
3961 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
4021 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
4081 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
4141 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
4201 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
4261 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
4321 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
4381 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
4441 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
4501 acaggaaaca gctatgacca tgattacgcc
//
pBI221-EGFP载体其他相关的植物过表达载体：
