pER8
pER8
编号 | 载体名称 |
北京华越洋VECT75580 | pER8 |
pER8载体基本信息：
启动子: | CaMV 35S |
复制子: | ori |
终止子: | NOS |
质粒分类: | 植物系列，蛋白过表达载体 |
质粒大小: | 11522bp |
原核抗性: | Spe |
筛选标记: | HygR |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 植物细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | 根据序列设计引物 |
pER8载体图谱：
pER8载体序列：
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
1141 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
1741 gagagctccc tggcgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
1801 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
1861 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
1921 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
1981 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
2041 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
2101 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
2161 gtaaaacatc ccaaacaagc ttggaaactg aaggcgggaa acgacaatct gatcatgagc
2221 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
2281 tttggaactg acagaaccgc aacgattgaa ggagccactc agccgcgggt ttctggagtt
2341 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
2401 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
2461 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatcccc
2521 tagaatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
2581 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
2641 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
2701 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
2761 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
2821 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
2881 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
2941 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
3001 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
3061 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
3121 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
3181 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
3241 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
3301 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
3361 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
3421 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
3481 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
3541 aaaggaatag cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
3601 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
3661 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
3721 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
3781 cggtgtcatc tatgttacta gatcggggaa ttgatccccc ctcgacagct tgcatgccag
3841 cttgggctgc aggtcgaggc taaaaaacta atcgcattat catcccctcg acgtactgta
3901 catataacca ctggttttat atacagcagt actgtacata taaccactgg ttttatatac
3961 agcagtcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
4021 accactggtt ttatatacag cagtcgaggt aagattagat atggatatgt atatggatat
4081 gtatatggtg gtaatgccat gtaatatgct cgactctagg atcttcgcaa gacccttcct
4141 ctatataagg aagttcattt catttggaga ggacacgctg aagctagtcg actctagcct
4201 cgaggcgcgc cgggcccagg cctacgcgtt taattaacta gtcgatccag gcctcccagc
4261 tttcgtccgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc
4321 ccacacacca gaatcctact aagtttgagt attatggcat tggaaaagct gttttcttct
4381 atcatttgtt ctgcttgtaa tttactgtgt tctttcagtt tttgttttcg gacatcaaaa
4441 tgcaaatgga tggataagag ttaataaatg atatggtcct tttgttcatt ctcaaattat
4501 tattatctgt tgtttttact ttaatgggtt gaatttaagt aagaaaggaa ctaacagtgt
4561 gatattaagg tgcaatgtta gacatataaa acagtctttc acctctcttt ggttatgtct
4621 tgaattggtt tgtttcttca cttatctgtg taatcaagtt tactatgagt ctatgatcaa
4681 gtaattatgc aatcaagtta agtacagtat aggctttttg tgtcgagggg gtaccgagtc
4741 gaggaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
4801 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
4861 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gggtaccgag ctcgaattca
4921 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
4981 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
5041 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
5101 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
5161 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
5221 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
5281 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
5341 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
5401 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
5461 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
5521 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
5581 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
5641 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
5701 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
5761 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
5821 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
5881 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
5941 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
6001 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
6061 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
6121 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
6181 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
6241 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
6301 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
6361 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
6421 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
6481 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
6721 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
7201 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
7261 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
7321 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
7381 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
7441 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
7501 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
7561 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
7621 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
7681 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
7741 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
7801 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
7861 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
7921 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
7981 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
8041 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
8101 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
8161 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
8221 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
8281 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
8341 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
8401 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
8461 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
8521 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
8581 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
8641 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
8701 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
8761 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
8821 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
8881 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
8941 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
9001 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
9061 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
9121 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
9181 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
9241 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
9301 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
9361 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
9421 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
9481 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
9541 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
9601 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
9661 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
9721 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
9781 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
9841 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
9901 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
9961 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
10021 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
10081 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
10141 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
10201 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
10261 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
10321 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
10381 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
10441 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
10501 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
10561 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
10621 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
10681 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
10741 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
10801 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
10861 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
10921 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
10981 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
11041 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
11101 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
11161 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
11221 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
11281 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
11341 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
11401 caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac
11461 ggataaacct tttcacgccc ttttaaatat ccgttattct aataaacgct cttttctctt
11521 ag
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