pIEX/Bac-1

pIEX/Bac-1

pIEXBac1载体基本信息

pIEXBac1载体质粒图谱和多克隆位点信息

pIEXBac1载体简介

The dual-purpose pIEx/Bac™ vectors are designed for cloning and high-level expression of proteins by transiently transfecting Spodoptera-derived insect cells or by generating baculovirus recombinants. Transient transfection and early baculovirus expression is driven by a promoter/enhancer combination that recruits endogenous insect cell transcription machinery, the AcNPV derived hr5 enhancer and ie1 promoter. Late/very late expression in the baculovirus mode is driven by the strong p10 promoter. The pIEx/Bac-1 vector carries an N-terminal Strep•Tag II coding sequence (1) followed by a recognition site for enterokinase.

The multiple cloning region is followed by an optional C-terminal His•Tag® coding sequence. The presence of two “gentle elution" tags at both the N- and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map.

pIEXBac1载体序列

ORIGIN     

          1 GGAACGGCTC CGCCCACTAT TAATGAAATT AAAAATTCCA ATTTTAAAAA ACGCAGCAAG

         61 AGAAACATTT GTATGAAAGA ATGCGTAGAA GGAAAGAAAA ATGTCGTCGA CATGCTGAAC

        121 AACAAGATTA ATATGCCTCC GTGTATAAAA AAAATATTGA ACGATTTGAA AGAAAACAAT

        181 GTACCGCGCG GCGGTATGTA CAGGAAGAGG TTTATACTAA ACTGTTACAT TGCAAACGTG

        241 GTTTCGTGTG CCAAGTGTGA AAACCGATGT TTAATCAAGG CTCTGACGCA TTTCTACAAC

        301 CACGACTCCA AGTGTGTGGG TGAAGTCATG CATCTTTTAA TCAAATCCCA AGATGTGTAT

        361 AAACCACCAA ACTGCCAAAA AATGAAAACT GTCGACAAGC TCTGTCCGTT TGCTGGCAAC

        421 TGCAAGGGTC TCAATCCTAT TTGTAATTAT TGAATAATAA AACAATTATA AATGCTAAAT

        481 TTGTTTTTTA TTAACGATAC AAACCAAACG CAACAAGAAC ATTTGTAGTA TTATCTATAA

        541 TTGAAAACGC GTAGTTATAA TCGCTGAGGT AATATTTAAA ATCATTTTCA AATGATTCAC

        601 AGTTAATTTG CGACAATATA ATTTTATTTT CACATAAACT AGACGCCTTG TCGTCTTCTT

        661 CTTCGTATTC CTTCTCTTTT TCATTTTTCT CTTCATAAAA ATTAACATAG TTATTATCGT

        721 ATCCATATAT GTATCTATCG TATAGAGTAA ATTTTTTGTT GTCATAAATA TATATGTCTT

        781 TTTTAATGGG GTGTATAGTA CCGCTGCGCA TAGTTTTTCT GTAATTTACA ACAGTGCTAT

        841 TTTCTGGTAG TTCTTCGGAG TGTGTTGCTT TAATTATTAA ATTTATATAA TCAATGAATT

        901 TGGGATCGTC GGTTTTGTAC AATATGTTGC CGGCATAGTA CGCAGCTTCT TCTAGTTCAA

        961 TTACACCATT TTTTAGCAGC ACCGGATTAA CATAACTTTC CAAAATGTTG TACGAACCGT

       1021 TAAACAAAAA CAGTTCACCT CCCTTTTCTA TACTATTGTC TGCGAGCAGT TGTTTGTTGT

       1081 TAAAAATAAC AGCCATTGTA ATGAGACGCA CAAACTAATA TCACAAACTG GAAATGTCTA

       1141 TCAATATATA GTTGCTGATC AGATCTGATC ATGGAGATAA TTAAAATGAT AAGGGCGCGT

       1201 AAAACACAAT CAAGTATGAG TCATAAGCTG ATGTCATGTT TTGCACACGG CTCATAACCG

       1261 AACTGGCTTT ACGAGTAGAA TTCTACTTGT AACGCACGAT CAGTGGATGA TGTCATTTGT

       1321 TTTTCAAATC GAGATGATGT CATGTTTTGC ACACGGCTCA TAAACTCGCT TTACGAGTAG

       1381 AATTCTACGT GTAACGCACG ATCGATTGAT GAGTCATTTG TTTTGCAATA TGATATCATA

       1441 CAATATGACT CATTTGTTTT TCAAAACCGA ACTTGATTTA CGGGTAGAAT TCTACTTGTA

       1501 AAGCACAATC AAAAAGATGA TGTCATTTGT TTTTCAAAAC TGAACTCGCT TTACGAGTAG

       1561 AATTCTACGT GTAAAACACA ATCAAGAAAT GATGTCATTT GTTATAAAAA TAAAAGCTGA

       1621 TGTCATGTTT TGCACATGGC TCATAACTAA ACTCGCTTTA CGGGTAGAAT TCTACGCGCG

       1681 TCGATGTCTT TGTGATGCGC GCGACATTTT TGTAGGTTAT TGATAAAATG AACGGATACG

       1741 TTGCCCGACA TTATCATTAA ATCCTTGGCG TAGAATTTGT CGGGTCCATT GTCCGTGTGC

       1801 GCTAGCATGC CCGTAACGGA CCTCGTACTT TTGGCTTCAA AGGTTTTGCG CACAGACAAA

       1861 ATGTGCCACA CTTGCAGCTC TGCATGTGTG CGCGTTACCA CAAATCCCAA CGGCGCAGTG

       1921 TACTTGTTGT ATGCAAATAA ATCTCGATAA AGGCGCGGCG CGCGAATGCA GCTGATCACG

       1981 TACGTCCCTC GTGTTCCGTT CAAGGACGGT GTTATCGACC TCAGATTAAT GTTTATCGGC

       2041 CGACTGTTTT CGTATCCGCT CACCAAACGC GTTTTTGCAT TAACATTGTA TGTCGGCGGA

       2101 TGTTCTATAT CTAATTTGAA TAAATAAACG ATAACCGCGT TGGTTTTAGA GGGCATAATA

       2161 AAAGAAATAT TGTTATCGTG TTCGCCATTA GGGCAGTATA AATTGACGTT CATGTTGGAT

       2221 ATTGTTTCAG TTGCAAGTTG ACACTGGCGG CGACAAGATC GTGAACAACC AAGTGACTCC

       2281 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC

       2341 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCCATGGCAA

       2401 GCTGGAGCCA CCCGCAGTTC GAAAAGGGTG CAGATGACGA CGACAAGGTA CCGGATCCGA

       2461 CGTCGGTCGA CAAGCTTGCG GCCGCAGGAG CTCCGGGCTT CTCCTCAATT TCCGCTCATC

       2521 ACCACCATCA TCACCATCAC CACCACTAAG TGATTAACCT CAGGTTATAC ATATATTTTG

       2581 AATTTAATTA ATTATACATA TATTTTATAT TATTTTTGTC TTTTATTATC GAGGGGCCGT

       2641 TGTTGGTGTG GGGTTTTGCA TAGAAATAAC AATGGGAGTT GGCGACGTTG CTGCGCCAAC

       2701 ACCACCTCCC TTCCCTCCTT TCATCATGTA TCTGTAGATA AAATAAAATA TTAAACCTAA

       2761 AAACAAGACC GCGCCTATCA ACAAAATGAT AGGCATTAAC TTGCCGCTGA CGCTGTCACT

       2821 AACGTTGGAC GATTTGCCGA CTAAACCTTC ATCGCCCAGT AACCAATCTA GGTAGCTGAG

       2881 CGCATGCAAG CTGATCCGGG TTATTAGTAC ATTTATTAAG CGCTAGATTC TGTGCGTTGT

       2941 TGATTTACAG ACAATTGTTG TACGTATTTT AATAATTCAT TAAATTTATA ATCTTTAGGG

       3001 TGGTATGTTA GAGCGAAAAT CAAATGATTT TCAGCGTCTT TATATCTGAA TTTAAATATT

       3061 AAATCCTCAA TAGATTTGTA AAATAGGTTT CGATTAGTTT CAAACAAGGG TTGTTTTTCC

       3121 GAACCGATGG CTGGACTATC TAATGGATTT TCGCTCAACG CCACAAAACT TGCCAAATCT

       3181 TGTAGCAGCA ATCTAGCTTT GTCGATATTC GTTTGTGTTT TGTTTTGTAA TAAAGGTTCG

       3241 ACGTCGTTCA AAATATTATG CGCTTTTGTA TTTCTTTCAT CACTGTCGTT AGTGTACAAT

       3301 TGACTCGACG TAAACACGTT AAATAGAGCT TGGACATATT TAACATCGGG CGTGTTAGCT

       3361 TTATTAGGCC GATTATCGTC GTCGTCCCAA CCCTCGTCGT TAGAAGTTGC TTCCGAAGAC

       3421 GATTTTGCCA TAGCCACACG ACGCCTATTA ATTGTGTCGG CTAACACGTC CGCGATCAAA

       3481 TTTGTAGTTG AGCTTTTTGG AATTATTTCT GATTGCGGGC GTTTTTGGGC GGGTTTCAAT

       3541 CTAACTGTGC CCGATTTTAA TTCAGACAAC ACGTTAGAAA GCGATGGTGC AGGCGGTGGT

       3601 AACATTTCAG ACGGCAAATC TACTAATGGC GGCGGTGGTG GAGCTGATGA TAAATCTACC

       3661 ATCGGTGGAG GCGCAGGCGG GGCTGGCGGC GGAGGCGGAG GCGGAGGTGG TGGCGGTGAT

       3721 GCAGACGGCG GTTTAGGCTC AAATGTCTCT TTAGGCAACA CAGTCGGCAC CTCAACTATT

       3781 GTACTGGTTT CGGGCGCCGT TTTTGGTTTG ACCGGTCTGA GACGAGTGCG ATTTTTTTCG

       3841 TTTCTAATAG CTTCCAACAA TTGTTGTCTG TCGTCTAAAG GTGCAGCGGG TTGAGGTTCC

       3901 GTCGGCATTG GTGGAGCGGG CGGCAATTCA GACATCGATG GTGGTGGTGG TGGTGGAGGC

       3961 GCTGGAATGT TAGGCACGGG AGAAGGTGGT GGCGGCGGTG CCGCCGGTAT AATTTGTTCT

       4021 GGTTTAGTTT GTTCGCGCAC GATTGTGGGC ACCGGCGCAG GCGCCGCTGG CTGCACAACG

       4081 GAAGGTCGTC TGCTTCGAGG CAGCGCTTGG GGTGGTGGCA ATTCAATATT ATAATTGGAA

       4141 TACAAATCGT AAAAATCTGC TATAAGCATT GTAATTTCGC TATCGTTTAC CGTGCCGATA

       4201 TTTAACAACC GCTCAATGTA AGCAATTGTA TTGTAAAGAG ATTGTCTCAA GCTCGGATCG

       4261 ATCCCGCACG CCGATAACAA GCCTTTTCAT TTTTACTACA GCATTGTAGT GGCGAGACAC

       4321 TTCGCTGTCG TCGAGGTTTA AACGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC

       4381 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG

       4441 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA

       4501 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC

       4561 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC

       4621 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG

       4681 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT

       4741 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC

       4801 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC

       4861 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG

       4921 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG

       4981 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA

       5041 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG

       5101 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT

       5161 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA

       5221 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT

       5281 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG

       5341 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA

       5401 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC

       5461 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT

       5521 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG

       5581 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA

       5641 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG

       5701 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA

       5761 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG

       5821 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT

       5881 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA

       5941 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA

       6001 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG

       6061 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC

       6121 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT

       6181 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC

       6241 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG

       6301 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC

       6361 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA

       6421 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC

       6481 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT

       6541 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA

       6601 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT

       6661 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA

       6721 CAATTTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT

       6781 CTTCGCTATT ACGCCA

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pIEX/Bac-1其他昆虫表达载体：