pCoBlast
pCoBlast
编号 | 载体名称 |
北京华越洋生物 KCVECT105 | pCoBlast |
pCoBlast载体基本信息
载体名称: | pCoBlast |
质粒类型: | 昆虫表达载体；筛选载体 |
克隆方法: | -- |
启动子: | copia |
载体大小: | 3907 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
真核筛选标记: | Blasticidin |
克隆菌株: | TOP10；DH5-T1R |
宿主细胞（系）: | 果蝇细胞系S2 |
备注: | pCoBlast载体与昆虫表达载体共转染，建立稳表达细胞系。 |
瞬表达/稳表达: | -- |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCoBlast载体质粒图谱和多克隆位点信息
pCoBlast载体简介
pCoBlast is a 3907 bp selection vector that can be cotransfected with the expression vector of choice to to create stable cell lines in Drosophila. It contains the Streptomyces griseochromogenes bsd gene under control of the Drosophila copia promoter to confer resistance to blasticidin in S2 cells.
引用及参考文献
A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.
Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;
J Biol Chem (2002) 277:15354-15416
Product usage: The full-length coding sequence of human TSG-6 (A431 allele encoding Gln at amino acid 144), including the signal sequence and stop codon, was excised from the pAcCL29-1 vector by digestion with KpnI/XbaI and cloned into the corresponding sites in the Drosophila Expression System
pCoBlast载体序列
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCT
GTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGG
CTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGAT
GCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATC
GGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTA
ACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTTGCATGCCTGCAGGTCG
ACTCTAGAGGATCCGGTGCGTGGTGGTTTCATGCTTCTGGGAACGGCAAATGGGTTTAGGATTGGGAACC
CCTCATCATCTGTTGGAATATACTATTCAACCTACAAAAATAACGTTAAACAACACTACTTTATATTTGA
TATGAATGGCCACACCTTTTATGCCATAAAACATATTGTAAGAGAATACCACTCTTTTTATTCCTTCTTT
CCTTCTTGTACGTTTTTTGCTGTGAGTAGGTCGTGGTGCTGGTGTTGCAGTTGAAATAACTTAAAATATA
AATCATAAAACTCAAACATAAACTTGACTATTTATTTATTTATTAAGAAAGGAAATATAAATTATAAATT
ACAACAGGTTCCCTTTATGCGAAGGGATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAG
AGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGC
GACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGCGCAGAACTCGTGGTGC
TGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGG
CATCTTGAGCCCCTGCGGACGGTGCCGACAGGTTCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTG
AAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGG
GCTAACCCTTATACGCAAGGGAGTAGATGCCGACCGAACAAGAGCTGATTTCGAGAACGCCTCAGCCAGC
AACTCGCGCGAGCCTAGCAAGGCAAATGCGAGAGAACGGCCTTACGCTTGGTGGCACAGTTCTCGTCCAC
AGTTCGCTAAGCTCGCTCGGCTGGGTCGCGGGAGGGCCGGTCGCAGTGATTCAGGCCCTTCTGGATTGTG
TTGGTCCCCAGGGCACGATTGTCATGCCCACGCACTCGGGTGATCTGACTGATCCCGCAGATTGGAGATC
GCCGCCCGTGCCTGCCGATTGGGTGCAGATCAGCCTCGAGGCCAGCTAGCTTGAACTTGTTTATTGCAGC
TTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCT
AGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCGGGTACCGAGCTCGAATTCGTA
ATCATGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAA
GCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCC
CGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGT
TTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAG
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACAT
GTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTC
CGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATA
CCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCG
GTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAA
CAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTAC
ACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAG
AAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCA
CGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAA
GTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC
ACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACG
ATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAG
ATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTC
CATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTT
GTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCC
AACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGAT
CGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACT
GTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTA
TGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAA
AGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGT
TCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAG
CAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACT
CTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGT
ATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAA
pCoBlast其他昆虫表达载体：
