pMIB/V5-His A
pMIB/V5-His A
编号 | 载体名称 |
北京华越洋生物 KCVECT134 | pMIB/V5-His A |
pMIB/V5-His A载体基本信息
载体名称: | pMIB/V5-His A |
质粒类型: | 昆虫表达载体；分泌表达载体 |
克隆方法: | 多克隆位点，限制性内切酶 |
启动子: | OplE2 |
载体大小: | 3596 bp |
5' 测序引物及序列: | OpIE2 Forward：CGCAACGATCTGGTAAACAC |
3' 测序引物及序列: | -- |
载体标签: | V5 Epitope Tag , His Tag (6x) |
载体抗性: | 氨苄青霉素 |
真核筛选标记: | Blasticidin |
克隆菌株: | TOP10 |
宿主细胞（系）: | Sf9, Sf21, High Five |
备注: | pMIB/V5-His A载体含HBM信号肽，让你的重组蛋白分泌型表达。 |
瞬表达/稳表达: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pMIB/V5-His A载体质粒图谱和多克隆位点信息
pMIB/V5-His A载体简介
pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:
• The OpIE2 promoter for constitutive expression
• The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin
引用及参考文献
Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals.
Bethony J, Loukas A, Smout M, Brooker S, Mendez S, Plieskatt J, Goud G, Bottazzi ME, Zhan B, Wang Y, Williamson A, Lustigman S, Correa-Oliveira R, Xiao S, Hotez PJ, FASEB J (2005) 19:1743-1745
Product usage: ASP-2 was fused in-frame into pMIB-V5/HisA using the SphI and XbaI restriction sites. Protein was expressed in Spodpotera frugiperda Sf9 insect cells.
pMIB/V5-His A载体序列
CATGATGATAAACAATGTATGGTGCTAATGTTGCTTCAACAACAATTCTGTTGAACTGTGTTTTCATGTT
TGCCAACAAGCACCTTTATACTCGGTGGCCTCCCCACCACCAACTTTTTTGCACTGCAAAAAAACACGCT
TTTGCACGCGGGCCCATACATAGTACAAACTCTACGTTTCGTAGACTATTTTACATAAATAGTCTACACC
GTTGTATACGCTCCAAATACACTACCACACATTGAACCTTTTTGCAGTGCAAAAAAGTACGTGTCGGCAG
TCACGTAGGCCGGCCTTATCGGGTCGCGTCCTGTCACGTACGAATCACATTATCGGACCGGACGAGTGTT
GTCTTATCGTGACAGGACGCCAGCTTCCTGTGTTGCTAACCGCAGCCGGACGCAACTCCTTATCGGAACA
GGACGCGCCTCCATATCAGCCGCGCGTTATCTCATGCGCGTGACCGGACACGAGGCGCCCGTCCCGCTTA
TCGCGCCTATAAATACAGCCCGCAACGATCTGGTAAACACAGTTGAACAGCATCTGTTCGAATTTAAAGC
TACCATGAAATTCTTAGTCAACGTTGCCCTTGTTTTTATGGTCGTATACATTTCTTACATCTATGCCGGC
ATGCTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCTGCAGATATCCAGCACAG
TGGCGGCCGCTCGAGTCTAGAGGGCCCTTCGAAGGTAAGCCTATCCCTAACCCTCTCCTCGGTCTCGATT
CTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTATCTGACTAAATCTTAGTTTGTATTGTCATG
TTTTAATACAATATGTTATGTTTAAATATGTTTTTAATAAATTTTATAAAATAATTTCAACTTTTATTGT
AACAACATTGTCCATTTACACACTCCTTTCAAGCGCGTGGGATCGATGCTCACTCAAAGGCGGTAATACG
GTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT
CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTG
GCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGT
AAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG
CGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC
TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCGAAACACGC
ACGGCGCGCGCACGCAGCTTAGCACAAACGCGTCGTTGCACGCGCCCACCGCTAACCGCAGGCCAATCGG
TCGGCCGGCCTCATATCCGCTCACCAGCCGCGTCCTATCGGGCGCGGCTTCCGCGCCCATTTTGAATAAA
TAAACGATAACGCCGTTGGTGGCGTGAGGCATGTAAAAGGTTACATCATTATCTTGTTCGCCATCCGGTT
GGTATAAATAGACGTTCATGTTGGTTTTTGTTTCAGTTGCAAGTTGGCTGCGGCGCGCGCAGCACCTTTG
CCGGGATCTGCCGGGCTGCAGCACGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATA
CGACAAGGTGAGGAACTAAACCATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCA
ACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACG
GCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGCGCAGAACTCGTGGTGCTGGG
CACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATC
TTGAGCCCCTGCGGACGGTGCCGACAGGTTCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGG
ACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTA
AGCACTTCGTGGCCGAGGAGCAGGACTGACACGTCCCGGGAGATCTGCATGTCTACTAAACTCACAAATT
AGAGCTTCAATTTAATTATATCAGTTATTACCCATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCG
TGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGA
TCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGC
GGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTG
CCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAAC
CGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTG
GCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC
ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT
CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGA
GTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTA
ACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC
TAGGTGAAGATCCTTTTTGATAATCT
pMIB/V5-His A其他昆虫表达载体：
