北京华越洋生物
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首页    载体及质粒    pPIC6 C
bjcn3scmz_6830_635929738735983031

pPIC6 C

pPIC6 C

pPIC6 C

编号

载体名称

北京华越洋生物VECT2320

pPIC6 C

 

pPIC6 C载体基本信息

载体名称:pPIC6 C
质粒类型:毕赤酵母表达载体;分泌表达载体;胞外表达载体
克隆方法:多克隆位点,限制性内切酶
启动子:AOX1
载体大小:3381 bp
5' 测序引物及序列:GACTGGTTCCAATTGACAAGC
3' 测序引物及序列:GCAAATGGCATTCTGACATCC
载体标签:c-Myc Epitope Tag, His Tag (6x)
载体抗性:Blasticidin
真核筛选标记:Blasticidin
克隆菌株:DH5α、TOP10、JM109
表达菌株:X-33
备注:pPIC6 C载体含E因子,让你的重组蛋白分泌型表达。
产品目录号:——
组成型/诱导型:诱导型(甲醇)

pPIC6 C载体质粒图谱和多克隆位点信息

pPIC6 C载体图谱



pPIC6 C 多克隆位点

pPIC6 C 载体特征

pPIC6 C载体简介


pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection,high-level expression, and rapid protein purification and detection. The pPIC6 A,B, & C vectors are designed for high-level secreted expression. Both sets of vectors have the following features:Blasticidin resistance for direct selection of multi-copy integrants Inducible AOX1 promoter for high-level expression in Pichia pastoris C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelatingresin and detection with an Anti-His(C-term) Antibody pPIC6 also features: An É -factor secretion signal for efficient transport of proteins to the medium.


载体序列

AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTC
ACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCAC
TCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCG
CTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTG
GCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGA
GGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCT
GTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCT
AACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGAC
GAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTG
TGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCC
AAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACT
TGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTT
ACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGA
AGATCAAAAAACAACTAATTATTCGAAACGAGGAATTCACGTGGCCCAGCCGGCCGTCTCGGATCGGTAC
CTCGAGCCGCGGCGGCCGCCAGCTTACGTAGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCG
TCGACCATCATCATCATCATCATTGAGTTTGTAGCCTTAGACATGACTGTTCCTCAGTTCAAGTTGGGCA
CTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGC
AGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCT
TCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAA
AATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAC
CTTCGTTTGTGCGGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGAT
TTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTT
TCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTC
TTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGT
TTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGT
TTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGAAAGCATAGCAATCTAAT
CTAAGGGGCGGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGA
ACTAAACCATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAA
CAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACT
GGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTG
CGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGG
ACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAG
CCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAAGCACTTCGTGGCC
GAGGAGCAGGACTGACACGTCCGACGGCGGCCCACGGGTCCCAGGCCTCGGAGATCCGTCCCCCTTTTCC
TTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCG
AAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAG
AACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTG
AAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGGAGACCAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC
CCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATAC
CAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTA
GGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGT
AACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAG
AAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGA
TCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTA
AGGGATTTTGGTCATGAGATC

pPIC6 C其他酵母表达载体:

pPIC6αA

pYD1

pGAG424

pSos-MAFB

pRS406

p53his

pGADT7-T

pBridge

pRS416

pCL1

pHis2

pACT2-AD

pSEP1

pSos

pGBKT7

pFLD/CAT

pHisSi-1

pGBKT7-53

pFLD

pYES2-EGFP

pMyr

pFLDα

Ycp22lac-EGFP

pPIC9k-His

pGBKT7-Lam

pADH2

SUMO protease

pPICZ A

pDisplay

pYIP5

pGADT7

LA503

pPinkα-HC

pDR195

BFD15

pPIC ZαFC

pPIC3.5K

pYES2

pPIC ZαGB

pYEPlac112

pAUR123

pPIC ZαD

pYEPlac195

pYRP7

pPICZ C

Ycplac33

pYX212

pFA6a-FGP(S65T)-kanMX6

pHIC-PI

pYIP211

pRS41H

pESC-URA

pRS426gal

pYES2-kan

pRS316

pYES6/CT

p416GFD

pPICZα B

pESC-His

pYES2-NTB

pYCP211

pESC-Leu

pYES2-NTA

pMET C

pRS426

pYES2-NTC

pMETαA

pPink-HC

pESC-TRP

pMETαB

pPink-LC

pGAPZ B

pYES3/CT

pMET A

pGAPZα C

pPICZα A

pMETαC

pHIL-S1

pPICZ B

pYC2/CT

pMET B

pPICZα C

pPIC9

pRS405

pHIL-D2

pPIC3.5

pGAPZ A

pRS414

pGAPZα A

pUG66

pAD-GAL4-2.1

pSEP2

pAO815

pDEST32

pYC2/NT B

p53blue

pGAPZ C

pYC2/NT A

pPIC6 C

pRS415

pYC2/NT C

pYES-DEST52

pPIC6αB

pSEP3

pYES2/CT

pPIC6αC

pBD-GAL4-Cam

pPIC6 A

pRS403

pRS413