pPinkα-HC
pPinkα-HC
pPinkα-HC
编号 | 载体名称 |
北京华越洋生物VECT2680 | pPinkα-HC |
pPinkα-HC载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pPinkalpha-HC, pPinkα-HC, pPinkalpha HC, pPinkα HC |
| 质粒类型: | 毕赤酵母蛋白表达载体（Pink酵母） |
| 高/低拷贝: | 高拷贝 |
| 启动子: | AOX1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7898 bp |
| 5' 测序引物及序列: | 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´ alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´ |
| 载体标签: | N-alpha 分泌信号肽 |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 拥有alpha因子，分泌表达蛋白。 |
| 产品目录号: | -- |
| 稳定性: | 稳定 Stable |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pPinkα-HC载体质粒图谱和多克隆位点信息
pPinkalpha-HC载体序列
LOCUS pPinkα-HC 7898 bp ds-DNA circular SYN 04-二月-2013 DEFINITION Pichia pastoris integrating vector for high-copy expression of a secreted protein in the PichiaPink(TM) system. ACCESSION . VERSION . KEYWORDS pPINK(alpha)-HC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct COMMENT This file is created by Vector NTI COMMENT Linearize within PpTRP2 for integration. FEATURES Location/Qualifiers source 1..7898 /organism="synthetic DNA construct" /lab_host="Pichia pastoris" /mol_type="other DNA" promoter 2..940 /gene="Pichia pastoris AOX1" /note="AOX1 promoter" /note="inducible promoter, regulated by methanol" /note="color: #ffffff; direction: RIGHT" CDS 941..1195 /codon_start=1 /gene="MF-alpha-1" /product="N-terminal secretion signal from S. cerevisiae alpha-factor (modified)" /note="alpha-factor secretion signal" /note="Cleavage by the Kex2 protease occurs after the dibasic KR sequence." /note="This feature has 2 segments: ???1:??941?..??997?/?#993366?/?presequence ???2:??998?..?1195?/?#993366?/?pro?region Cleavage site after base 997" /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR" misc_feature 1196..1222 /note="MCS" /note="multiple cloning site" /note="color: #99ccff" terminator 1248..1497 /gene="S. cerevisiae CYC1" /note="CYC1 terminator" /note="transcription terminator for CYC1" /note="color: #ffffff" promoter 1517..1529 /gene="Pichia pastoris ADE2" /note="PpADE2 HC promoter" /note="weak 13-bp promoter fragment that requires high-copy integration for efficient expression" /note="color: #ffffff; direction: RIGHT" CDS 1530..3221 /codon_start=1 /gene="Pichia pastoris ADE2" /product="phosphoribosylaminoimidazole carboxylase from Pichia pastoris" /note="PpADE2" /note="essential for the biosynthesis of purine nucleotides, including adenine" /note="color: #ff7f50" promoter 3448..3597 /gene="Pichia pastoris TRP2" /note="PpTRP2 promoter" /note="promoter for Pichia pastoris TRP2" /note="color: #ffffff; direction: RIGHT" CDS 3598..5214 /codon_start=1 /gene="Pichia pastoris TRP2" /product="anthranilate synthase from Pichia pastoris" /note="PpTRP2" /note="catalyzes the initial step of tryptophan biosynthesis" /note="color: #ff7f50" rep_origin complement(5678..6266) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /note="color: #ffff00" CDS complement(6437..7297) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(7298..7402) /gene="bla" /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" ORIGIN 1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt 961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga 1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga 1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa 1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaaggcctca 1201 ggtaccggcc ggccatttaa atacaggccc cttttccttt gtcgatatca tgtaattagt 1261 tatgtcacgc ttacattcac gccctcctcc cacatccgct ctaaccgaaa aggaaggagt 1321 tagacaacct gaagtctagg tccctattta ttttttttaa tagttatgtt agtattaaga 1381 acgttattta tatttcaaat ttttcttttt tttctgtaca aacgcgtgta cgcatgtaac 1441 attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag 1501 ctggatccgc ggccgccttc caaactctca tggattctca ggtaataggt attctaggag 1561 gaggccagct aggccgaatg attgttgagg ccgctagcag gctcaatatc aagaccgtga 1621 ttcttgatga tggtttttca cctgctaagc acattaatgc tgcgcaagac cacatcgacg 1681 gatcattcaa agatgaggag gctatcgcca agttagctgc caaatgtgat gttctcactg 1741 tagagattga gcatgtcaac acagatgctc taaagagagt tcaagacaga actggaatca 1801 agatatatcc tttaccagag acaatcgaac taatcaagga taagtacttg caaaaggaac 1861 atttgatcaa gcacaacatt tcggtgacaa agtctcaggg tatagaatct aatgaaaagg 1921 cgctgctttt gtttggagaa gagaatggat ttccatatct gttgaagtcc cggactatgg 1981 cttatgatgg aagaggcaat tttgtagtgg agtctaaaga ggacatcagt aaggcattag 2041 agttcttgaa agatcgtcca ttgtatgccg agaagtttgc tccttttgtt aaagaattag 2101 cggtaatggt tgtgagatca ctggaaggcg aagtattctc ctacccaacc gtagaaactg 2161 tgcacaagga caatatctgt catattgtgt atgctccggc cagagttaat gacaccatcc 2221 aaaagaaagc tcaaatatta gctgaaaaca ctgtgaagac tttcccaggc gctggaatct 2281 tcggagttga gatgttccta ttgtctgatg gagaacttct tgtaaatgag attgctccaa 2341 ggccccacaa ttctggtcac tatacaatcg atgcatgtgt aacatctcag ttcgaagcac 2401 atgtaagagc cataactggt ctgccaatgc cactagattt caccaaacta tctacttcca 2461 acaccaacgc tattatgctc aatgttttgg gtgctgaaaa atctcacggg gaattagagt 2521 tttgtagaag agccttagaa acacccggtg cttctgtata tctgtacgga aagaccaccc 2581 gattggctcg taagatgggt catatcaaca taataggatc ttccatgttg gaagcagaac 2641 aaaagttaga gtacattcta gaagaatcaa cccacttacc atccagtact gtatcagctg 2701 acactaaacc gttggttgga gttatcatgg gttcagactc tgatctacct gtgatttcga 2761 aaggttgcga tattttaaaa cagtttggtg ttccattcga agttactatt gtctctgctc 2821 atagaacacc acagagaatg accagatatg cctttgaagc cgctagtaga ggtatcaagg 2881 ctatcattgc aggtgctggt ggtgctgctc atcttccagg aatggttgct gccatgactc 2941 cgttgccagt cattggtgtt cctgtcaagg gctctacgtt ggatggtgta gactcgctac 3001 actcgattgt ccaaatgcct agaggtgttc ctgtggctac ggttgctatc aacaacgcca 3061 ccaatgccgc tctgttggcc atcaggattt taggtacaat tgaccacaaa tggcaaaagg 3121 aaatgtccaa gtatatgaat gcaatggaga ccgaagtgtt ggggaaggca tccaacttgg 3181 aatctgaagg gtatgaatcc tatttgaaga atcgtctttg aatttagtat tgttttttaa 3241 tagatgtata tataatagta cacgtaactt atctattcca ttcataattt tattttaaag 3301 gttcggtaga aatttgtcct ccaaaaagtt ggttagagcc tggcagtttt gataggcatt 3361 attatagatt gggtaatatt taccctgcac ctggaggaac tttgcaaaga gcctcatgtg 3421 cggcgcgcca ggccataatg gccaaacggt ttctcaatta ctatatacta ctaaccattt 3481 acctgtagcg tatttctttt ccctcttcgc gaaagctcaa gggcatcttc ttgactcatg 3541 aaaaatatct ggatttcttc tgacagatca tcacccttga gcccaactct ctagcctatg 3601 agtgtaagtg atagtcatct tgcaacagat tattttggaa cgcaactaac aaagcagata 3661 cacccttcag cagaatcctt tctggatatt gtgaagaatg atcgccaaag tcacagtcct 3721 gagacagttc ctaatcttta ccccatttac aagttcatcc aatcagactt cttaacgcct 3781 catctggctt atatcaagct taccaacagt tcagaaactc ccagtccaag tttcttgctt 3841 gaaagtgcga agaatggtga caccgttgac aggtacacct ttatgggaca ttcccccaga 3901 aaaataatca agactgggcc tttagagggt gctgaagttg accccttggt gcttctggaa 3961 aaagaactga agggcaccag acaagcgcaa cttcctggta ttcctcgtct aagtggtggt 4021 gccataggat acatctcgta cgattgtatt aagtactttg aaccaaaaac tgaaagaaaa 4081 ctgaaagatg ttttgcaact tccggaagca gctttgatgt tgttcgacac gatcgtggct 4141 tttgacaatg tttatcaaag attccaggta attggaaacg tttctctatc cgttgatgac 4201 tcggacgaag ctattcttga gaaatattat aagacaagag aagaagtgga aaagatcagt 4261 aaagtggtat ttgacaataa aactgttccc tactatgaac agaaagatat tattcaaggc 4321 caaacgttca cctctaatat tggtcaggaa gggtatgaaa accatgttcg caagctgaaa 4381 gaacatattc tgaaaggaga catcttccaa gctgttccct ctcaaagggt agccaggccg 4441 acctcattgc accctttcaa catctatcgt catttgagaa ctgtcaatcc ttctccatac 4501 atgttctata ttgactatct agacttccaa gttgttggtg cttcacctga attactagtt 4561 aaatccgaca acaacaacaa aatcatcaca catcctattg ctggaactct tcccagaggt 4621 aaaactatcg aagaggacga caattatgct aagcaattga agtcgtcttt gaaagacagg 4681 gccgagcacg tcatgctggt agatttggcc agaaatgata ttaaccgtgt gtgtgagccc 4741 accagtacca cggttgatcg tttattgact gtggagagat tttctcatgt gatgcatctt 4801 gtgtcagaag tcagtggaac attgagacca aacaagactc gcttcgatgc tttcagatcc 4861 attttcccag caggaaccgt ctccggtgct ccgaaggtaa gagcaatgca actcatagga 4921 gaattggaag gagaaaagag aggtgtttat gcgggggccg taggacactg gtcgtacgat 4981 ggaaaatcga tggacacatg tattgcctta agaacaatgg tcgtcaagga cggtgtcgct 5041 taccttcaag ccggaggtgg aattgtctac gattctgacc cctatgacga gtacatcgaa 5101 accatgaaca aaatgagatc caacaataac accatcttgg aggctgagaa aatctggacc 5161 gataggttgg ccagagacga gaatcaaagt gaatccgaag aaaacgatca atgaacggag 5221 gacgtaagta ggaatttatg gtttggccat aatggcctag cttggcgtaa tcatggtcat 5281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 5341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 5401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 5461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 6721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 7021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 7501 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 7561 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 7621 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 7681 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 7741 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 7801 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 7861 cccagtcacg acgttgtaaa acgacggcca gtgaattg
pPinkα-HC其他酵母表达载体：
