pESC-His
pESC-His
pESC-His
编号 | 载体名称 |
北京华越洋生物VECT2220 | pESC-His |
pESC-His载体基本信息
| 载体名称: | pESC-His, pESC His |
|---|---|
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1和GAL10 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6706 bp |
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
| 载体标签: | C-Flag, C-Myc |
| 载体抗性: | 氨苄 |
| 筛选标记: | His3 |
| 备注: | 利用半乳糖诱导，可以同时使两个基因在酿酒酵母中表达。 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pESC-His载体质粒图谱和多克隆位点信息
pESC-His载体序列
LOCUS pESC-HIS 6706 bp DNA circular SYN 16-SEP-2005 DEFINITION Cloning vector pESC-HIS, complete sequence. ACCESSION AF063850 VERSION AF063850.1 GI:3228560 SOURCE Cloning vector pESC-HIS. ORGANISM Cloning vector pESC-HIS artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..6706 /organism="Cloning vector pESC-HIS" /mol_type="genomic DNA" /specific_host="Escherichia coli K12" /db_xref="taxon:76667" /vntifkey="98" promoter 2556..3010 /vntifkey="29" /label=GAL1\Promoter promoter complement(2344..2555) /vntifkey="29" /label=GAL10\promoter CDS 504..1160 /vntifkey="4" /label=HIS3 /note="yeast HIS3 ORF" rep_origin complement(1555..1861) /vntifkey="33" /label=f1\ori terminator complement(1961..2125) /vntifkey="43" /label=ADH1\terminator misc_feature complement(2256..2339) /vntifkey="21" /label=MCS1 misc_feature complement(2274..2300) /vntifkey="21" /label=FLAG\tag misc_feature 3012..3087 /vntifkey="21" /label=MCS2 misc_feature 3052..3087 /vntifkey="21" /label=c-myc\tag terminator 3114..3303 /vntifkey="43" /label=CYC1\terminator rep_origin 3490..4157 /vntifkey="33" /label=pUC\ori CDS complement(4308..5165) /vntifkey="4" /label=AmpR rep_origin 5299..6454 /vntifkey="33" /label=2\micron\ori BASE COUNT 1868 a 1467 c 1480 g 1891 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc 1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa 2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata 2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg 2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg 2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg 2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg 2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt 2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat 2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt 2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg 2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt 2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact 2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat 2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct 2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa 2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca 2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga 3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag 3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct 3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 3481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 3541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 3601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 3661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 3721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 3781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 3841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 3901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 3961 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 4021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 4081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 4141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 4201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 4261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 4321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 4381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 4441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 4501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 4561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 4621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 4681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 4741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 4801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 4861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 4921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 4981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 5041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 5101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 5161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5281 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 5341 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 5401 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 5461 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 5521 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 5581 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 5641 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 5701 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 5761 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 5821 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 5881 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 5941 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 6001 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 6061 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 6121 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 6181 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 6241 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 6301 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 6361 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 6421 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 6481 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 6541 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 6601 ctcatccttc aatgctatca tttcctttga tattggatca tctaagaaac cattattatc 6661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc //
pESC-His其他酵母表达载体：
