PX855
PX855
编号 | 载体名称 |
北京华越洋VECT75258 | PX855 |
PX855载体基本信息：
载体名称: | PX855 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | -- |
插入位点: | Age1，EcoR1 |
启动子: | CBh |
载体大小: | 6205 bp |
5' 测序引物及序列: | ctggagcacctgcctgaaatcact |
3' 测序引物及序列: | GGCTGATCAGCGAGCTCTAG |
载体标签: | NES、PTK2(N ter)；FRB、NES、PTK2(C ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞等 |
备注: | A portion of this plasmid was derived from a plasmid made by SpCas9 piece was PCR amplified of PX481 (dCas9) |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
PX855 载体质粒图谱和多克隆位点信息：
PX855 载体序列：
ORIGIN
1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt GTGGAAAGGA
241 CGAAACACCg gGTCTTCgaG AAGACctgtt ttagagctaG AAAtagcaag ttaaaataag
301 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcTTTTTTg ttttagagct
361 agaaatagca agttaaaata aggctagtcc gtTTTTagcg cgtgcgccaa ttctgcagac
421 aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
481 ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc cattgacgtc
541 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
601 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tGtgcccagt
661 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
721 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
781 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
841 ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga
901 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc
961 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgacgc
1021 tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg gctctgactg
1081 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
1141 ctgagcaaga ggtaagggtt taagggatgg ttggttggtg gggtattaat gtttaattac
1201 ctggagcacc tgcctgaaat cacttttttt caggttGGac cggtgccacc ATGCTAGATT
1261 TAGCTAGCTT AATCCTCGAC AAGAAGTACA GCATCGGCCT GGcCATCGGC ACCAACTCTG
1321 TGGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCAG CAAGAAATTC AAGGTGCTGG
1381 GCAACACCGA CCGGCACAGC ATCAAGAAGA ACCTGATCGG AGCCCTGCTG TTCGACAGCG
1441 GCGAAACAGC CGAGGCCACC CGGCTGAAGA GAACCGCCAG AAGAAGATAC ACCAGACGGA
1501 AGAACCGGAT CTGCTATCTG CAAGAGATCT TCAGCAACGA GATGGCCAAG GTGGACGACA
1561 GCTTCTTCCA CAGACTGGAA GAGTCCTTCC TGGTGGAAGA GGATAAGAAG CACGAGCGGC
1621 ACCCCATCTT CGGCAACATC GTGGACGAGG TGGCCTACCA CGAGAAGTAC CCCACCATCT
1681 ACCACCTGAG AAAGAAACTG GTGGACAGCA CCGACAAGGC CGACCTGCGG CTGATCTATC
1741 TGGCCCTGGC CCACATGATC AAGTTCCGGG GCCACTTCCT GATCGAGGGC GACCTGAACC
1801 CCGACAACAG CGACGTGGAC AAGCTGTTCA TCCAGCTGGT GCAGACCTAC AACCAGCTGT
1861 TCGAGGAAAA CCCCATCAAC GCCAGCGGCG TGGACGCCAA GGCCATCCTG TCTGCCAGAC
1921 TGAGCAAGAG CAGACGGCTG GAAAATCTGA TCGCCCAGCT GCCCGGCGAG AAGAAGAATG
1981 GCCTGTTCGG AAACCTGATT GCCCTGAGCC TGGGCCTGAC CCCCAACTTC AAGAGCAACT
2041 TCGACCTGGC CGAGGATGCC AAACTGCAGC TGAGCAAGGA CACCTACGAC GACGACCTGG
2101 ACAACCTGCT GGCCCAGATC GGCGACCAGT ACGCCGACCT GTTTCTGGCC GCCAAGAACC
2161 TGTCCGACGC CATCCTGCTG AGCGACATCC TGAGAGTGAA CACCGAGATC ACCAAGGCCC
2221 CCCTGAGCGC CTCTATGATC AAGAGATACG ACGAGCACCA CCAGGACCTG ACCCTGCTGA
2281 AAGCTCTCGT GCGGCAGCAG CTGCCTGAGA AGTACAAAGA GATTTTCTTC GACCAGAGCA
2341 AGAACGGCTA CGCCGGCTAC ATTGACGGCG GAGCCAGCCA GGAAGAGTTC TACAAGTTCA
2401 TCAAGCCCAT CCTGGAAAAG ATGGACGGCA CCGAGGAACT GCTCGTGAAG CTGAACAGAG
2461 AGGACCTGCT GCGGAAGCAG CGGACCTTCG ACAACGGCAG CATCCCCCAC CAGATCCACC
2521 TGGGAGAGCT GCACGCCATT CTGCGGCGGC AGGAAGATTT TTACCCATTC CTGAAGGACA
2581 ACCGGGAAAA GATCGAGAAG ATCCTGACCT TCCGCATCCC CTACTACGTG GGCCCTCTGG
2641 CCAGGGGAAA CAGCAGATTC GCCTGGATGA CCAGAAAGAG CGAGGAAACC ATCACCCCCT
2701 GGAACTTCGA GGAAGTGGTG GACAAGGGCG CTTCCGCCCA GAGCTTCATC GAGCGGATGA
2761 CCAACTTCGA TAAGAACCTG CCCAACGAGA AGGTGCTGCC CAAGCACAGC CTGCTGTACG
2821 AGTACTTCAC CGTGTATAAC GAGCTGACCA AAGTGAAATA CGTGACCGAG GGAATGAGAG
2881 GTGGCGGTGG CtcgGGTGGC GGTGGCtcgG GTGGCGGTGG CtcgGAGATG TGGCACGAGG
2941 GCCTGGAGGA GGCCAGCAGA CTGTACTTCG GCGAGAGAAA CGTGAAGGGC ATGTTCGAGG
3001 TGCTGGAGCC CCTGCACGCC ATGATGGAGA GAGGCCCCCA GACCCTGAAG GAGACCAGCT
3061 TCAACCAGGC CTACGGCAGA GACCTGATGG AGGCCCAGGA GTGGTGCAGA AAGTACATGA
3121 AGAGCGGCAA CGTGAAGGAC CTGACCCAGG CCTGGGACCT GTACTACCAC GTGTTCAGAA
3181 GAATCAGCAA GCAGCTAGAT TTAGCTAGCT TAATCCTCta agaattcCTA GAGCTCGCTG
3241 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
3301 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC
3361 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA
3421 GGGGGAGGAT TGGGAAGAgA ATAGCAGGCA TGCTGGGGAg cggccgcagg aacccctagt
3481 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
3541 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
3601 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
3661 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
3721 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
3781 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
3841 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
3901 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
3961 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
4021 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
4081 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4141 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4201 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
4261 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
4321 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
4381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
4441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
4501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
4561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
4621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
4681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
4741 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
4801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
4861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
4921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
4981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5041 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
5221 tctggagccg gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag
5281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
5341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6181 ttttgctggc cttttgctca catgt
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PX855载体其他相关CRISPR/Cas9载体：
