北京华越洋生物
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pX603

pX603

@pX603

 

pX603



 

编号

载体名称

北京华越洋VECT75222

pX603

 

 

pX603 载体基本信息:

载体名称:

pX603 pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

质粒类型:

CRISPR-Cas9载体;基因敲除载体;哺乳动物细胞载体

高拷贝/低拷贝:

高拷贝

插入位点:

限制性内切酶,多克隆位点

启动子:

CMV

载体大小:

7101 bp

5' 测序引物及序列:

CMV Forward:CGCAAATGGGCGGTAGGCGTG

3' 测序引物及序列:

BGH ReverseTAGAAGGCACAGTCGAGG

载体标签:

3xHA (N ter)

载体抗性:

氨苄青霉素

筛选标记:

--

克隆菌株:

Stbl3

宿主细胞(系):

哺乳动物细胞

备注:

--

稳定性:

瞬表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pX603载体质粒图谱和多克隆位点信息:

 

 

pX603载体序列:

ORIGIN

    1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt

   61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt

  121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc

  181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa

  241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct

  301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta

  361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg

  421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc

  481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta

  541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg

  601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt

  661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg

  721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta

  781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg

  841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg

  901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc

  961 ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg

 1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg

 1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag

 1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat

 1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc

 1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc

 1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa

 1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac

 1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt

 1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc

 1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa

 1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca

 1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat

 1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa

 1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc

 1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca

 1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc

 1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag

 2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg

 2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc

 2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga

 2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg

 2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag

 2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga

 2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg

 2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg

 2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa

 2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg

 2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg

 2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcctcg aggcgttgac

 2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat

 2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg

 2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt

 2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag

 3001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc

 3061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag

 3121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt

 3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc

 3241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg

 3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actaccggtg

 3361 ccaccATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCAAGC

 3421 GGAACTACAT CCTGGGCCTG GcCATCGGCA TCACCAGCGT GGGCTACGGC ATCATCGACT

 3481 ACGAGACACG GGACGTGATC GATGCCGGCG TGCGGCTGTT CAAAGAGGCC AACGTGGAAA

 3541 ACAACGAGGG CAGGCGGAGC AAGAGAGGCG CCAGAAGGCT GAAGCGGCGG AGGCGGCATA

 3601 GAATCCAGAG AGTGAAGAAG CTGCTGTTCG ACTACAACCT GCTGACCGAC CACAGCGAGC

 3661 TGAGCGGCAT CAACCCCTAC GAGGCCAGAG TGAAGGGCCT GAGCCAGAAG CTGAGCGAGG

 3721 AAGAGTTCTC TGCCGCCCTG CTGCACCTGG CCAAGAGAAG AGGCGTGCAC AACGTGAACG

 3781 AGGTGGAAGA GGACACCGGC AACGAGCTGT CCACCAAAGA GCAGATCAGC CGGAACAGCA

 3841 AGGCCCTGGA AGAGAAATAC GTGGCCGAAC TGCAGCTGGA ACGGCTGAAG AAAGACGGCG

 3901 AAGTGCGGGG CAGCATCAAC AGATTCAAGA CCAGCGACTA CGTGAAAGAA GCCAAACAGC

 3961 TGCTGAAGGT GCAGAAGGCC TACCACCAGC TGGACCAGAG CTTCATCGAC ACCTACATCG

 4021 ACCTGCTGGA AACCCGGCGG ACCTACTATG AGGGACCTGG CGAGGGCAGC CCCTTCGGCT

 4081 GGAAGGACAT CAAAGAATGG TACGAGATGC TGATGGGCCA CTGCACCTAC TTCCCCGAGG

 4141 AACTGCGGAG CGTGAAGTAC GCCTACAACG CCGACCTGTA CAACGCCCTG AACGACCTGA

 4201 ACAATCTCGT GATCACCAGG GACGAGAACG AGAAGCTGGA ATATTACGAG AAGTTCCAGA

 4261 TCATCGAGAA CGTGTTCAAG CAGAAGAAGA AGCCCACCCT GAAGCAGATC GCCAAAGAAA

 4321 TCCTCGTGAA CGAAGAGGAT ATTAAGGGCT ACAGAGTGAC CAGCACCGGC AAGCCCGAGT

 4381 TCACCAACCT GAAGGTGTAC CACGACATCA AGGACATTAC CGCCCGGAAA GAGATTATTG

 4441 AGAACGCCGA GCTGCTGGAT CAGATTGCCA AGATCCTGAC CATCTACCAG AGCAGCGAGG

 4501 ACATCCAGGA AGAACTGACC AATCTGAACT CCGAGCTGAC CCAGGAAGAG ATCGAGCAGA

 4561 TCTCTAATCT GAAGGGCTAT ACCGGCACCC ACAACCTGAG CCTGAAGGCC ATCAACCTGA

 4621 TCCTGGACGA GCTGTGGCAC ACCAACGACA ACCAGATCGC TATCTTCAAC CGGCTGAAGC

 4681 TGGTGCCCAA GAAGGTGGAC CTGTCCCAGC AGAAAGAGAT CCCCACCACC CTGGTGGACG

 4741 ACTTCATCCT GAGCCCCGTC GTGAAGAGAA GCTTCATCCA GAGCATCAAA GTGATCAACG

 4801 CCATCATCAA GAAGTACGGC CTGCCCAACG ACATCATTAT CGAGCTGGCC CGCGAGAAGA

 4861 ACTCCAAGGA CGCCCAGAAA ATGATCAACG AGATGCAGAA GCGGAACCGG CAGACCAACG

 4921 AGCGGATCGA GGAAATCATC CGGACCACCG GCAAAGAGAA CGCCAAGTAC CTGATCGAGA

 4981 AGATCAAGCT GCACGACATG CAGGAAGGCA AGTGCCTGTA CAGCCTGGAA GCCATCCCTC

 5041 TGGAAGATCT GCTGAACAAC CCCTTCAACT ATGAGGTGGA CCACATCATC CCCAGAAGCG

 5101 TGTCCTTCGA CAACAGCTTC AACAACAAGG TGCTCGTGAA GCAGGAAGAA gccagCAAGA

 5161 AGGGCAACCG GACCCCATTC CAGTACCTGA GCAGCAGCGA CAGCAAGATC AGCTACGAAA

 5221 CCTTCAAGAA GCACATCCTG AATCTGGCCA AGGGCAAGGG CAGAATCAGC AAGACCAAGA

 5281 AAGAGTATCT GCTGGAAGAA CGGGACATCA ACAGGTTCTC CGTGCAGAAA GACTTCATCA

 5341 ACCGGAACCT GGTGGATACC AGATACGCCA CCAGAGGCCT GATGAACCTG CTGCGGAGCT

 5401 ACTTCAGAGT GAACAACCTG GACGTGAAAG TGAAGTCCAT CAATGGCGGC TTCACCAGCT

 5461 TTCTGCGGCG GAAGTGGAAG TTTAAGAAAG AGCGGAACAA GGGGTACAAG CACCACGCCG

 5521 AGGACGCCCT GATCATTGCC AACGCCGATT TCATCTTCAA AGAGTGGAAG AAACTGGACA

 5581 AGGCCAAAAA AGTGATGGAA AACCAGATGT TCGAGGAAAA GCAGGCCGAG AGCATGCCCG

 5641 AGATCGAAAC CGAGCAGGAG TACAAAGAGA TCTTCATCAC CCCCCACCAG ATCAAGCACA

 5701 TTAAGGACTT CAAGGACTAC AAGTACAGCC ACCGGGTGGA CAAGAAGCCT AATAGAGAGC

 5761 TGATTAACGA CACCCTGTAC TCCACCCGGA AGGACGACAA GGGCAACACC CTGATCGTGA

 5821 ACAATCTGAA CGGCCTGTAC GACAAGGACA ATGACAAGCT GAAAAAGCTG ATCAACAAGA

 5881 GCCCCGAAAA GCTGCTGATG TACCACCACG ACCCCCAGAC CTACCAGAAA CTGAAGCTGA

 5941 TTATGGAACA GTACGGCGAC GAGAAGAATC CCCTGTACAA GTACTACGAG GAAACCGGGA

 6001 ACTACCTGAC CAAGTACTCC AAAAAGGACA ACGGCCCCGT GATCAAGAAG ATTAAGTATT

 6061 ACGGCAACAA ACTGAACGCC CATCTGGACA TCACCGACGA CTACCCCAAC AGCAGAAACA

 6121 AGGTCGTGAA GCTGTCCCTG AAGCCCTACA GATTCGACGT GTACCTGGAC AATGGCGTGT

 6181 ACAAGTTCGT GACCGTGAAG AATCTGGATG TGATCAAAAA AGAAAACTAC TACGAAGTGA

 6241 ATAGCAAGTG CTATGAGGAA GCTAAGAAGC TGAAGAAGAT CAGCAACCAG GCCGAGTTTA

 6301 TCGCCTCCTT CTACAACAAC GATCTGATCA AGATCAACGG CGAGCTGTAT AGAGTGATCG

 6361 GCGTGAACAA CGACCTGCTG AACCGGATCG AAGTGAACAT GATCGACATC ACCTACCGCG

 6421 AGTACCTGGA AAACATGAAC GACAAGAGGC CCCCCAGGAT CATTAAGACA ATCGCCTCCA

 6481 AGACCCAGAG CATTAAGAAG TACAGCACAG ACATTCTGGG CAACCTGTAT GAAGTGAAAT

 6541 CTAAGAAGCA CCCTCAGATC ATCAAAAAGG GCAAAAGGCC GGCGGCCACG AAAAAGGCCG

 6601 GCCAGGCAAA AAAGAAAAAG ggatccTACC CATACGATGT TCCAGATTAC GCTTATCCCT

 6661 ACGACGTGCC TGATTATGCA TACCCATATG ATGTCCCCGA CTATGCCTAA gaattcctag

 6721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc

 6781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga

 6841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca

 6901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagC TAGAggccgc

 6961 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg

 7021 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc

 7081 gagcgcgcag ctgcctgcag g

//



 

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