pGenesil-1
pGenesil-1
pGenesil-1
编号 | 载体名称 |
北京华越洋VECT75621 | pGenesil-1 |
pGenesil-1载体基本信息：
启动子: | hU6,SV40,CMV |
复制子: | pUC ori，SV40 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒干扰载体 |
质粒大小: | 4893bp |
质粒标签: | EGFP |
原核抗性: | Kan |
筛选标记: | Neo |
克隆菌株: | Stbl3 |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | CMV-F：CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
pGenesil-1载体图谱：
pGenesil-1载体序列：
The pGenesil-1 plasmid was obtained by the transformation of pEGFP-C1 plasmid. PEGFP-C1 plasmid has been widely used in mammalian cells for many years, it has fluorescent protein expression, it is very convenient to detect the effect of. When your target gene was not detected by conventional fluorescence detection, the pGenesil-1 plasmid encoding shRNAs was a better choice.
pGenesil-1载体其他相关的慢病毒载体：
