pCFB
pCFB
编号 | 载体名称 |
北京华越洋VECT55130 | pCFB |
pCFB逆病毒载体基本信息：
载体名称: | pCFB |
质粒类型: | 逆病毒载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 5292 bp |
5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | 无 |
克隆菌株: | DH5α 等通用菌株 |
宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 逆转录病毒 |
pCFB载体质粒图谱和多克隆位点信息：
pCFB载体简介：
pCFB载体限制性酶切位点
pCFB, 5292 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 6 328, 381, 464, 650, 1153
5218
Acc65I 3 822, 2021, 2818
AccI 3 2142, 2232, 3178
AflII 3 202, 1440, 2373
AflIII 2 164, 3407
AgeI 1 2015
AhdI 6 862, 908, 1449, 2858, 2904
4295
Alw44I 4 1229, 3223, 3721, 4967
AlwNI 3 2494, 2569, 3818
ApaI 1 1413
ApoI 3 87, 1303, 2238
AvaI 8 785, 818, 1416, 2193, 2249
2748, 2781, 2814
BamHI 1 2244
BanII 5 939, 1413, 2751, 2764, 2935
BbeI 3 790, 1831, 2786
BbsI 1 5285
BbvCI 1 2624
BciVI 5 831, 2161, 2827, 3616, 5143
BglI 4 291, 413, 484, 4414
BglII 1 1853
BpmI 3 1974, 2321, 4385
Bpu10I 4 1723, 2508, 2583, 2624
BsaAI 4 545, 1991, 2175, 3159
BsaHI 10 328, 381, 464, 650, 790
1153, 1831, 2786, 4836, 5218
BsaI 9 869, 890, 957, 1589, 1977
2865, 2886, 2953, 4367
BsaWI 5 138, 2015, 3613, 3760, 4591
BseRI 5 893, 1243, 1736, 1775, 2889
BsiEI 8 1136, 1209, 2013, 2255, 3320
3744, 4667, 4816
BsiHKAI 6 1229, 2751, 3223, 3721, 4882
4967
BsmBI 6 1151, 1268, 1512, 1571, 1757
3056
Bsp120I 1 1413
BspHI 3 4127, 5135, 5240
BspLU11I 2 164, 3407
BsrBI 5 1474, 2252, 2277, 3338, 5139
BsrDI 2 4354, 4536
BsrGI 1 1716
BssHII 1 2734
BssSI 3 3580, 4964, 5271
Bst1107I 1 3178
BstAPI 3 2493, 2568, 3225
BstEII 1 1521
BstF5I 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
Bsu36I 1 1451
Cfr10I 2 2015, 4380
DraI 3 4164, 4183, 4875
DraIII 1 2048
DrdI 2 3096, 3509
DsaI 3 151, 567, 1106
EaeI 7 1002, 1136, 1543, 1564, 1843
2255, 4688
EarI 4 1509, 1739, 3285, 5089
Ecl136II 1 2751
Eco52I 2 1136, 2255
Eco57I 2 3934, 4982
Eco72I 1 2175
EcoNI 1 1822
EcoO109I 4 1652, 2099, 2670, 5275
EcoRI 1 2238
EcoRV 2 2479, 2554
EheI 3 790, 1831, 2786
FokI 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
FspI 1 4520
HaeII 5 790, 1831, 2786, 3281, 3651
HgaI 7 724, 1064, 1341, 3113, 3509
4087, 4837
HincII 2 2232, 4839
KasI 3 790, 1831, 2786
KpnI 3 822, 2021, 2818
MboII 10 1509, 1740, 2271, 3286, 4057
4148, 4903, 4981, 5090, 5286
MscI 3 1002, 1543, 1843
MslI 5 159, 568, 4548, 4707, 5066
MspA1I 9 151, 918, 2457, 2532, 2914
3117, 3747, 3992, 4933
MunI 2 11, 21
NarI 3 790, 1831, 2786
NcoI 1 567
NdeI 4 440, 1839, 1847, 3229
NheI 5 6, 16, 26, 197, 2368
NotI 1 2254
NsiI 1 774
NspI 4 164, 244, 3042, 3407
PleI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
Ppu10I 1 774
PpuMI 3 1652, 2099, 2670
PshAI 1 1190
Psp1406I 2 4525, 4898
PstI 3 1353, 1535, 4541
PvuI 2 1209, 4667
PvuII 2 2457, 2532
SacI 1 2751
SacII 1 151
SalI 1 2232
SapI 1 3284
ScaI 1 4778
SchI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
SexAI 1 1649
SfcI 7 182, 1353, 1535, 2353, 3672
3863, 4541
SmaI 4 818, 1416, 2193, 2814
SmlI 8 202, 1440, 2249, 2373, 3513
3775, 4052, 4920
SnaBI 1 545
SpeI 1 1072
SphI 1 244
SrfI 1 1415
SspI 1 5102
StyI 5 567, 881, 1679, 2667, 2877
TaqI 9 1472, 1783, 1794, 2225, 2233
2250, 2294, 3507, 4951
TatI 7 424, 504, 537, 588, 1716
3213, 4778
TfiI 1 3382
Tsp45I 6 1457, 1666, 3062, 3157, 4557
4768
TspRI 10 1459, 2050, 3303, 3809, 3822
4093, 4242, 4347, 4694, 4721
Tth111I 4 808, 1648, 2804, 3151
VspI 1 4471
XbaI 2 2211, 2635
XhoI 1 2249
XhoII 8 1853, 2244, 4048, 4059, 4145
4157, 4925, 4942
XmaI 4 818, 1416, 2193, 2814
XmnI 1 4895
Enzymes that do NOT cut molecule:
AccIII AscI BclI BlnI
Bpu1102I BsaBI BsgI BsiWI
BsmI BspMI BstBI BstXI
ClaI Eco47III FseI HindIII
HpaI MluI NaeI NgoMIV
NruI PacI PmeI RsrII
SanDI SbfI SfiI SgfI
SgrAI StuI SwaI Van91I
XcmI
pCFB载体序列：
pCFB, 5292 bp version 013001
NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been
obtained from existing data.
1 GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG
51 AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT
101 CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG
151 CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
201 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACGCATGCC
251 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG
301 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC
351 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG
451 TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
501 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
551 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG
601 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG
651 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG
751 GTGGGAGGTC TATATAAGCA GAGATGCATC CTCACTCGGG GCGCCAGTCC
801 TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG
851 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG
901 AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG
951 GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC GGGAGGTAAG
1001 CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
1051 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG
1101 CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG
1151 GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC
1201 AAAAATCCCG ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGAGGG
1251 ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT
1301 CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC GCGTCTTGTC
1351 TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT
1401 GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA
1451 CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA
1501 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC
1551 CTTTAACGTC GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA
1601 CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG ACACCCAGAC
1651 CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC
1701 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT
1751 CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC
1801 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCCCCA TATGGCCATA
1851 TGAGATCTTA TATGGGGCAC CCCCGCCCCT TGTAAACTTC CCTGACCCTG
1901 ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA CTTACAGGCT
1951 CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CACGTACCAA
2001 GAACAACTGG ACCGACCGGT GGTACCTCAC CCTTACCGAG TCGGCGACAC
2051 AGTGTGGGTC CGCCGACACC AGACTAAGAA CCTAGAACCT CGCTGGAAAG
2101 GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA AGTAGACGGC
2151 ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG
2201 TGGACCATCC TCTAGACTGC CGGATCGAAT TGTCGACGAA TTCGGATCCT
2251 CGAGCGGCCG CGATCCGAGT TCTTCTGAGC GGGACTCTGG GGTTCGATAA
2301 AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC
2351 ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG
2401 GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG
2451 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC
2501 TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA
2551 CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG
2601 ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG
2651 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA
2701 ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
2751 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG
2801 ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT
2851 TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG
2901 ATTGACTACC CGTCAGCGGG GGTCTTTCAT TTGGGGGCTC GTCCGGGATC
2951 GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA GGTAAGCTGG
3001 CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
3051 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA
3101 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
3151 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC
3201 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC
3251 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG
3301 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC
3351 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
3401 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
3451 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
3501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
3551 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
3601 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
3651 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
3701 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
3751 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
3801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
3851 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
3901 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
3951 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
4001 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
4051 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
4101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
4151 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
4201 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
4251 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
4301 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
4351 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
4401 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
4451 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
4501 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT
4551 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
4601 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
4651 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
4701 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
4751 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
4801 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA
4851 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
4901 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
4951 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
5001 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
5051 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
5101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
5151 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
5201 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
5251 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AA
pCFB其他相关逆病毒载体：
