pBARGPE1
pBARGPE1
编号 | 载体名称 |
北京华越洋VECT75453 | pBARGPE1 |
pBARGPE1载体基本信息：
启动子: | gpdA，TrpC |
复制子: | pUC ori |
终止子: | trpC |
质粒分类: | 广宿主穿梭载体；真菌过表达质粒 |
质粒大小: | 5518bp |
原核抗性: | 氨苄青霉素Ampicillin |
筛选标记: | 草胺膦Bar |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 丝状真菌 |
诱导方式: | 无需诱导 |
5'测序引物: | 根据全序列设计 |
3'测序引物: | 根据全序列设计 |
备注: | 图谱中带下划线的是单一酶切位点 |
pBARGPE1载体图谱：
pBARGPE1载体简介：
pBARGPE1是一个丝状真菌-大肠杆菌穿梭表达载体，利用gpdA promoter可启动外源目的基因的表达，转化至丝状真菌中可用草胺膦Bar筛选。
pBARGPE1载体序列：
ORIGIN
1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
61 cccgggggat cctctagagt cgaggtcgac ggtatcgata agcttgatta aaggttcttg
121 gatgggaaga tgaatatact gaagatggga aaagaaagag aaaagaaaag agcagctggt
181 ggggagagca ggaaaatatg gcaacaaatg ttggactgac gcaacgacct tgtcaacccc
241 gccgacacac cgggcggaca gacggggcaa agctgcctac cagggactga gggacctcag
301 caggtcgagt gcagagcacc ggatgggtcg actgccagct tgtgttcccg gtctgcgccg
361 ctggccagct cctgagcggc ctttccggtt tcatacaccg ggcaaagcag gagaggcacg
421 atatttggac gccctacaga tgccggatgg gccaattagg gagcttacgc gccgggtact
481 cgctctacct acttcggaga aggtactatc tcgtgaatct tttaccagat cggaagcaat
541 tggacttctg tacctaggtt aatggcatgc tatttcgccg acggctatac acccctggct
601 tcacattctc cttcgcttac tgccggtgat tcgatgaagc tccatattct ccgatgatgc
661 aatagattct tggtcaacga ggggcacacc agcctttcca cttcggggcg gaggggcggc
721 cggtcccgga ttaataatca tccactgcac ctcagagccg ccagagctgt ctggcgcagt
781 ggcgcttatt actcagccct tctctctgcg tccgtccgtc tctccgcatg ctttgcatac
841 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagag
901 atctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
961 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
1021 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
1081 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
1141 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
1201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
1261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
1321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
1381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
1441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
1501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
1561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
1621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
1681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
1741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
1801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
1861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
1921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
1981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
2041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
2101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
2161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
2221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
2281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
2341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
2401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
2461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
2521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
2581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
2641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
2701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
2761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
2821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
2881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
2941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
3001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
3061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
3121 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
3181 gccctttcgt cttcaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
3241 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
3301 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
3361 cagagcacag aggccgcaga atgtgctcta gactcgacag aagatgatat tgaaggagca
3421 ctttttgggc ttggctggag ctagtggagg tcaacaatga atgcctattt tggtttagtc
3481 gtccaggcgg tgagcacaaa atttgtgtcg tttgacaaga tggttcattt aggcaactgg
3541 tcagatcagc cccacttgta gcagtagcgg cggcgctcga agtgtgactc ttattagcag
3601 acaggaacga ggacattatt atcatctgct gcttggtgca cgataacttg gtgcgtttgt
3661 caagcaaggt aagtgaacga cccggtcata ccttcttaag ttcgcccttc ctccctttat
3721 ttcagattca atctgactta cctattctac ccaagcaaag cttcgattag gaagtaacca
3781 tgagcccaga acgacgcccg gccgacatcc gccgtgccac cgaggcggac atgccggcgg
3841 tctgcaccat cgtcaaccac tacatcgaga caagcacggt caacttccgt accgagccgc
3901 aggaaccgca ggagtggacg gacgacctcg tccgtctgcg ggagcgctat ccctggctcg
3961 tcgccgaggt ggacggcgag gtcgccggca tcgcctacgc gggcccctgg aaggcacgca
4021 acgcctacga ctggacggcc gagtcgaccg tgtacgtctc cccccgccac cagcggacgg
4081 gactgggctc cacgctctac acccacctgc tgaagtccct ggaggcacag ggcttcaaga
4141 gcgtggtcgc tgtcatcggg ctgcccaacg acccgagcgt gcgcatgcac gaggcgctcg
4201 gatatgcccc ccgcggcatg ctgcgggcgg ccggcttcaa gcacgggaac tggcatgacg
4261 tgggtttctg gcagctggac ttcagcctgc cggtaccgcc ccgtccggtc ctgcccgtca
4321 ccgagatctg atccgtcgac ctgcagatcg ttcaaacatt tggcaataaa gtttcttaag
4381 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
4441 gcatgtaata attaacatgt aatgcatagt accgagaaac tagtggatct aaacataaaa
4501 tctgtaaaat aacaagatgt aaagataatg ctaaatcatt tggctttttg attgattgta
4561 caggaaaata tacatcgcag ggggttgact tttaccattt caccgcaatg gaatcaaact
4621 tgttgaagag aatgttcaca ggcgcatacg ctacaatgac ccgattcttg ctagcctttt
4681 ctcggtcttg caaacaaccg ccgcggccgg tcgagggacc taataacttc gtatagcata
4741 cattatacga agttatatta agggttccgg atcgcggccg ctcgacctgc agccaagcta
4801 gcttggctgg acgtaaactc ctcttcagac ctaataactt cgtatagcat acattatacg
4861 aagttatatt aagggttatt gaatatgatc ggaattggtc gatcaggtat tgctgttatc
4921 tgatgagtat acgttgtcct ggccacggca gaagcacgct tatcgctcca atttcccaca
4981 acattagtca actccgttag gcccttcatt gaaagaaatg aggtcatcaa atgtcttcca
5041 atgtgagatt ttgggccatt ttttatagca aagattgaat aaggcgcatt tttcttcaaa
5101 gcttgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
5161 tgttacatgc gtacacgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg
5221 ggactctaat cataaaaacc catctcataa ataacgtcat gcattgcaga tgagctgtat
5281 ctggaagagg taaacccgaa acgcgtttta ttcttgttga catggagcta ttaaatcact
5341 agaaggcact ctttgctgct tggacaaatg aacgtatctt atcgagatcc tgaacaccat
5401 ttgtctcaac tccggagctg acatcgacac caacgatctt atatccagat tcgtcaagct
5461 gtttgatgat ttcagtaacg ttaagtggat ctcggtgacg ggcaggaccg gacggggc
//
pBARGPE1载体其他相关的乳酸菌载体：
