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首页    载体及质粒    pBMTB-4
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pBMTB-4

pBMTB-4

pBMTB-4



 

编号

载体名称

北京华越洋VECT75131

pBMTB-4

 

pBMTB-4载体基本信息:

载体名称:

pBMTB-4

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

5363 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

四环素

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBMTB-4载体质粒图谱和多克隆位点信息:

 

pBMTB-4载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt

      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct

      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct

      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa

      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc

      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt

      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt

      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt

      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct

      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct

      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct

      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg

      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga

      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt

      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc

      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt

     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca

     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt

     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg

     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc

     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc

     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt

     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag

     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc

     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc

     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg

     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc

     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt

     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc

     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg

     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa

     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc

     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt

     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta

     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa

     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc

     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa

     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt

     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg

     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga

     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga

     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca

     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc

     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc

     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt

     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc

     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgaa

     2881 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg

     2941 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc

     3001 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt

     3061 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg

     3121 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg

     3181 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga

     3241 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt

     3301 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc

     3361 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca

     3421 ggagtcgcat aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc

     3481 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat

     3541 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg

     3601 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc

     3661 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc

     3721 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat

     3781 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc

     3841 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc

     3901 tcttaccagc ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc

     3961 ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct

     4021 ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagggatccc

     4081 cctcaagtca aaagcctccg gtcggaggct tttgactttc tgctatggag gtcaggtatg

     4141 atttttatga caacttgacg gctacatcat tcactttttc ttcacaaccg gcacggaact

     4201 cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt tgatcgtcaa

     4261 aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg

     4321 cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac tgctggcgga

     4381 aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa

     4441 aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc cgattatcca

     4501 tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca

     4561 gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta atgatttgcc

     4621 caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg

     4681 caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga aagtaaaccc

     4741 actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc tctcctggcg

     4801 ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt ttcaccaccc

     4861 cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt cggtcgataa

     4921 aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa

     4981 acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc atactcccgc

     5041 cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac tgcgtctttt

     5101 actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt ctgtaacaaa

     5161 gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac ggcagaaaag

     5221 tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt ttatccataa

     5281 gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct ccatacccgt

     5341 ttttttgggc tagagaattc gtc

//

 

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