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pCS22+

pCS22+

pCS22+



 

编号

载体名称

北京华越洋VECT75138

pCS22+

 

pCS22+载体基本信息:

载体名称:

pCS22+

质粒类型:

克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

simian CMV IE94lacT3SP6

载体大小:

4046 bp

5' 测序引物及序列:

SP6 fwdMCS1)、T3 fwdMCS2

3' 测序引物及序列:

EBV revMCS1)、SV40 polyA revMCS2

载体标签:

载体抗性:

氨苄青霉素

克隆菌株:

DH10B

表达细胞:

原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。

备注:

pCS22+载体能够在多种细胞中进行高水平的瞬表达。pCS22+载体同时也能进行体外转录和翻译。

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

pCS22+载体质粒图谱和多克隆位点信息:

pCS22+载体序列:

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag ctttaatacg actcactata gggcaataca

       61 agctacttgt ttaaactctg cagggatccg aattcctcga gccctttagt gagggttaat

      121 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat

      181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat

      241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca

      301 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta

      361 ccgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca

      421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg

      481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg

      541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc

      601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta

      661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag

      721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg

      781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg

      841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg

      901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga

      961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc

     1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt

     1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact

     1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg

     1201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt

     1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt

     1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct

     1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg

     1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt

     1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt

     1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc

     1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg

     1681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc

     1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg

     1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca

     1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga

     1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct

     1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg

     2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca

     2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata

     2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct

     2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact

     2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa

     2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc

     2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga

     2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga

     2521 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt

     2581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag

     2641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga

     2701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg

     2761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc

     2821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg

     2881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc

     2941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat

     3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt

     3061 cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct

     3121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct

     3181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt

     3241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct

     3301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt

     3361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct

     3421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt

     3481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt

     3541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc

     3601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa

     3661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt

     3721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc

     3781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag

     3841 tacattacta ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc

     3901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa

     3961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct

     4021 atataagcaa tgctcgttta gggaac

//

 

pCS22+其他相关广宿主载体

pMHE3Tc

pMHE7Tc

pMHE7

pMHE6

pMHE5Tc

pMHE3

pMHE2

pBBR1MCS-6

pMHE5

pBMTB-5

pBMTB-4

pBMTB-3

pBMTB-2

pBMTB-1

pBTL-5

pBTL-4

pBTL-3

pBTL-2

pBTL-1

pBTB-5

pBTB-4

pBTB-3

pBTB-2

pBTB-1

pBT-5

pBMT-5

pBMT-4

pBMT-3

pBMT-2

pBMT-1

pBT-4

pBT-2

pBT-3

pBT-1

pCS2+MT

pCS2+8CmCherry

pCS2+8CeGFP

pMMB207

pCS22+

pCS2+

pCS2-

pCS111

pCS108

pCS107

pBBR1MCS

pBBR1MCS-5

pBBR1MCS-4

pBBR1MCS-3

pBBR1MCS-2

pCSDest

pSPORT2

pBBR1-GFP

pCS2+8