钙黄绿素，Calcein
钙黄绿素，Calcein
编码 | 品名 | 规格 | 单位 |
北京华越洋生物 GX3541-1g | 钙黄绿素，Calcein | 1g | 瓶 |
北京华越洋生物 GX3541-10g | 钙黄绿素，Calcein | 10g | 瓶 |
实验耗材：钙黄绿素
【名 称】：钙黄绿素
【英 文】：Calcein
【分子式】：C30H24N2Na2O13
【分子量】：666.51
【性 状】：橙色结晶性粉末，有黄带绿色的荧光。溶于水，不溶于无水乙醇和乙醚。
钙黄绿素应密封干燥保存。
钙黄绿素（Calcein），也就是大家所熟知的fluorescein（钙黄绿素）、荧光素复合物；是一种激发光波长在495nm处，发射光波长在515nm处的钙离子荧光指示剂。在其浓度大于100 mM时，钙黄绿色荧光自我淬灭。它常被用做络合物指示剂，用来滴定EDTA螯合的钙离子，并且用荧光检测法测定钙离子浓度。它具有非常好的水溶性，其质子解离常数（酸度系数）pKa1=2。1， pKa2=2。9， pKa3=4。2， pKa4=5。5， pKa5=10。8和pKa6=11。7。Calcein-AM是一种可对活细胞进行荧光标记的细胞染色试剂，Calcein-AM由于在Calcein（钙黄绿素）的基础上加强了疏水性，因此能够轻易穿透活细胞膜。当其进入到细胞质后，酯酶会将其水解为Calcein（钙黄绿素）留在细胞内，发出强绿色荧光。与其它同类试剂（如BCECF-AM和Carboxy-fluorescein diacetate）相比，Calcein-AM是最适合作为荧光 探针去染活细胞的，因为它的细胞毒性很低。
钙黄绿素，Calcein英文注释： Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to calcein. The acetomethoxy (AM) derivate of calcein (calcein AM) is widely used for labeling live cells as it can be transported through the cellular membrane into live cells. The AM ester groups mask the part of the molecule that chelates calcium. Upon transporting into live cells cellular esterases cut off the AM groups, the molecule binds to calcium within cell (resulting in acquiring strong green fluorescence), and gets trapped inside. As dead cells lack esterases, only live cells are marked. This feature makes it very useful for testing of cell viability and for short-term marking of cells. Compared with other live cell-labeling reagents (such as BCECF-AM and carboxy-fluorescein diacetate), calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity. Calcein does not significantly affect cellular functions such as proliferation or chemotaxis of lymophocyte. In addition, viability assays using calcein are reliable and correlate well with the standard 51Cr-release assay.
钙黄绿素，Calcein
另外在核酸扩增过程中会形成大量的焦磷酸根离子。荧光染料中的锰离子与钙黄绿素结合导致荧光淬灭，同时染料颜色为橙色。当扩增反应形成焦磷酸根离子时，锰离子与焦磷酸根离子结合形成焦磷酸锰，引起荧光信号的产生，同时染料颜色变为黄绿色。钙黄绿素目前已广泛应用于恒温扩增反应的荧光检测。
钙黄绿素，Calcein现货促销
