pHANNIBAL
pHANNIBAL
pHANNIBAL
产品编号 | 载体名称 |
北京华越洋生物VECT0490 | pHANNIBAL |
pHANNIBAL载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pHANNIBAL |
| 质粒类型: | 植物表达载体 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
pHANNIBAL载体质粒图谱和多克隆位点信息
pHANNIBAL载体简介
LOCUS AJ311872 5824 bp DNA circular SYN 14-NOV-2006 DEFINITION Cloning vector pHANNIBAL. ACCESSION AJ311872 VERSION AJ311872.1 GI:15982214 KEYWORDS AMP gene; ampicillin resistance protein; pdk gene; promoter. SOURCE Cloning vector pHANNIBAL ORGANISM Cloning vector pHANNIBAL other sequences; artificial sequences; vectors. REFERENCE 1 AUTHORS Wesley,S.V., Helliwell,C.A., Smith,N.A., Wang,M.B., Rouse,D.T., Liu,Q., Gooding,P.S., Singh,S.P., Abbott,D., Stoutjesdijk,P.A., Robinson,S.P., Gleave,A.P., Green,A.G. and Waterhouse,P.M. TITLE Construct design for efficient, effective and high-throughput gene silencing in plants JOURNAL Plant J. 27 (6), 581-590 (2001) PUBMED 11576441REFERENCE 2 (bases 1 to 5824) AUTHORS Waterhouse,P.M. TITLE Direct Submission JOURNAL Submitted (04-MAY-2001) Waterhouse P.M., Plant Industry, C.S.I.R.O., P.O. Box 1600, Canberra, ACT 2601, AUSTRALIAFEATURES Location/Qualifiers source 1..5824 /organism="Cloning vector pHANNIBAL" /mol_type="other DNA" /db_xref="taxon:167047" /lab_host="Escherichia coli" /focus /note="pHANNIBAL is a derivative of cloning vector pART7 which was a derivative of pGEM-9Zf(-)" source 1..2864 /organism="Escherichia coli K-12" /mol_type="other DNA" /strain="K-12" /db_xref="taxon:83333" source 2865..4210 /organism="Cauliflower mosaic virus" /mol_type="other DNA" /db_xref="taxon:10641" source 4254..4995 /organism="Flaveria trinervia" /mol_type="other DNA" /db_xref="taxon:4227" source 5049..5814 /organism="Agrobacterium tumefaciens" /mol_type="other DNA" /db_xref="taxon:358" gene 775..1635 /gene="amp" CDS 775..1635 /gene="amp" /codon_start=1 /transl_table=11 /product="ampicillin resistance protein" /protein_id="CAC86250.1" /db_xref="GI:15982215" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 2865..4210 /regulatory_class="promoter" /function="35S promoter" gene 4254..4995 /gene="pdk" intron 4254..4995 /gene="pdk" /note="from pyruvate orthophosphate dikinase (pdk)" /number=2 regulatory 5049..5814 /regulatory_class="terminator" /note="octopine esynthase (ocs) terminator"ORIGIN 1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat 181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg 661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc 2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc 2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat 2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga 3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt 3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga 3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc 3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag 3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct 3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac 3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag 3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat 3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa 3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca 3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc 3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg 3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa 3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag 3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc 3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg 3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg 4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc 4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg 4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg 4201 agaggacacg ctcgaggaat tcggtacccc aattggtaag gaaataatta ttttcttttt 4261 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt 4321 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa 4381 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat 4441 ttttaatgaa tttgatcgaa catgtaagat gatatactag cattaatatt tgttttaatc 4501 ataatagtaa ttctagctgg tttgatgaat taaatatcaa tgataaaata ctatagtaaa 4561 aataagaata aataaattaa aataatattt ttttatgatt aatagtttat tatataatta 4621 aatatctata ccattactaa atattttagt ttaaaagtta ataaatattt tgttagaaat 4681 tccaatctgc ttgtaattta tcaataaaca aaatattaaa taacaagcta aagtaacaaa 4741 taatatcaaa ctaatagaaa cagtaatcta atgtaacaaa acataatcta atgctaatat 4801 aacaaagcgc aagatctatc attttatata gtattatttt caatcaacat tcttattaat 4861 ttctaaataa tacttgtagt tttattaact tctaaatgga ttgactatta attaaatgaa 4921 ttagtcgaac atgaataaac aaggtaacat gatagatcat gtcattgtgt tatcattgat 4981 cttacatttg gattgattac agttgggaaa ttgggttcga aatcgataag cttggatcct 5041 ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca 5101 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt 5161 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct 5221 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat 5281 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat 5341 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa 5401 gactgattac ataaatctta ttcaaatttc aaaaggcccc aggggctagt atctacgaca 5461 caccgagcgg cgaactaata acgttcactg aagggaactc cggttccccg ccggcgcgca 5521 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc 5581 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag 5641 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac 5701 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct 5761 gcaggcatgc aagctagctt actagtgatg catattctat agtgtcacct aaatctgcgg 5821 ccgc//
pHANNIBAL其他植物表达载体：
