pCambia1391Z

pCambia1391Z

pCambia 1391Z载体基本信息

pCambia 1391Z载体质粒图谱和多克隆位点信息

pCambia 1391Z载体序列

LOCUS       pCAMBIA1391Z          11227 bp ds-DNA    circular SYN 

DEFINITION  Agrobacterium binary vector for plant transformation, with 

            hygromycin- and kanamycin-resistance and LacZ-GUS genes plus the 

            pUC9 MCS.

ACCESSION   AF234312

VERSION     .

KEYWORDS    pCAMBIA1391Z

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 11227)

  AUTHORS   Cambia

  TITLE     Direct Submission

  JOURNAL   Exported from SnapGene Viewer 

COMMENT     The GenBank record was corrected by inserting a G at position 4743.

FEATURES             Location/Qualifiers

     source          1..11227

                     /organism="synthetic DNA construct"

                     /lab_host="Plant Cells"

                     /mol_type="other DNA"

     CDS             join(2..16,207..2024)

                     /codon_start=1

                     /product="beta-glucuronidase"

                     /label="GUS"

                     /label="This version of the gusA gene has a 5' extension 

                     with a catalase intron to ensure expression in plants but 

                     not bacteria."

                     /protein_id="

                     "                    

     intron          17..206

                     /label="cat1 intron"

                     /label="castor bean catalase intron, modified"

     CDS             2031..2048

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label="6xHis"

                     /translation="HHHHHH"

     terminator      2083..2335

                     /label="NOS terminator"

                     /label="nopaline synthase terminator and poly(A) signal"

     misc_feature    2357..2381

                     /label="RB T-DNA repeat"

                     /label="right border repeat from nopaline C58 T-DNA"

     CDS             3681..4310

                     /codon_start=1

                     /product="stability protein from Pseudomonas plasmid pVS1"

                     /label="pVS1 StaA"                     

     CDS             4739..5812

                     /codon_start=1

                     /product="replication protein from Pseudomonas plasmid 

                     pVS1"

                     /label="pVS1 RepA"

                     /label="

                     "

                     /protein_id="

                     "                     

     misc_feature    6416..6556

                     /label="bom"

                     /label="basis of mobility region from pBR322"

     rep_origin      complement(6742..7330)

                     /direction=LEFT

                     /label="ori"

                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7417..8211)

                     /codon_start=1

                     /gene="aphA-3"

                     /product="aminoglycoside phosphotransferase"

                     /label="KanR"

                     /label="confers resistance to kanamycin"

                     /protein_id="

                     "

     misc_feature    8636..8660

                     /label="LB T-DNA repeat"

                     /label="left border repeat from nopaline C58 T-DNA"

     polyA_signal    8738..8912

                     /label="CaMV poly(A) signal"

                     /label="cauliflower mosaic virus polyadenylation signal"

     CDS             complement(8952..9977)

                     /codon_start=1

                     /product="hygromycin B phosphotransferase"

                     /label="HygR"

                     /label="confers resistance to hygromycin"                     

     promoter        complement(10045..10722)

                     /label="CaMV 35S promoter (enhanced)"

                     /label="cauliflower mosaic virus 35S promoter with a 

                     duplicated enhancer region"

     promoter        10936..10966

                     /label="lac promoter"

                     /label="promoter for the E. coli lac operon"

     protein_bind    10974..10990

                     /bound_moiety="lac repressor encoded by lacI"

                     /label="lac operator"

                     /label="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     primer_bind     10998..11014

                     /label="M13 rev"

                     /label="common sequencing primer, one of multiple similar 

                     variants"

     CDS             11010..11207

                     /codon_start=1

                     /gene="lacZ (fragment)"

                     /product="LacZ-alpha fragment of?beta-galactosidase"

                     /label="lacZ-alpha"

                     /protein_id="

                     "

                     /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF

                     ASWRNSEEARTDRPSQQLRSL"

     misc_feature    11027..11062

                     /label="MCS"

                     /label="multiple cloning site from pUC9"

     primer_bind     complement(11063..11079)

                     /label="M13 fwd"

                     /label="common sequencing primer, one of multiple similar 

                     variants"

ORIGIN

        1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct

       61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga

      121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt

      181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa

      241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg

      301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag

      361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt

      421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg

      481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg

      541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac

      601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga

      661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg

      721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg

      781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca

      841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag

      901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc

      961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg

     1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct

     1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg

     1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct

     1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga

     1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca

     1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact

     1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga

     1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg

     1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct

     1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt

     1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac

     1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata

     1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat

     1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat

     1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag

     1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga

     1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc

     2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg

     2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt

     2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga

     2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata

     2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa

     2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt

     2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg

     2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg

     2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca

     2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc

     2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa

     2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt

     2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat

     2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg

     2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga

     2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc

     3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt

     3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg

     3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct

     3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc

     3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg

     3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga

     3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt

     3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac

     3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc

     3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta

     3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa

     3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag

     3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc

     3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc

     3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg

     3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg

     3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg

     4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac

     4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg

     4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct

     4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg

     4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg

     4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag

     4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt

     4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac

     4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat

     4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag

     4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg

     4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt

     4741 gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt

     4801 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc

     4861 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc

     4921 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt

     4981 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt

     5041 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg

     5101 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt

     5161 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga

     5221 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc

     5281 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca

     5341 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg

     5401 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat

     5461 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt

     5521 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg

     5581 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga

     5641 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg

     5701 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct

     5761 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga

     5821 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt

     5881 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg

     5941 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa

     6001 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct

     6061 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct

     6121 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg

     6181 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc

     6241 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg

     6301 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg

     6361 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag

     6421 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga

     6481 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag

     6541 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt

     6601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc

     6661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa

     6721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa

     6781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc

     6841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc

     6901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag

     6961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga

     7021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc

     7081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac

     7141 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg

     7201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca

     7261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa

     7321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa

     7381 ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat

     7441 ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac

     7501 atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca

     7561 cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac

     7621 aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc

     7681 cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt

     7741 ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct

     7801 gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca

     7861 ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca

     7921 aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg

     7981 caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc

     8041 cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt

     8101 ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg

     8161 gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca tttattattt

     8221 ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac

     8281 tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag

     8341 ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga

     8401 tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc

     8461 cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca

     8521 aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg

     8581 tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca

     8641 ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt

     8701 ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt

     8761 ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact

     8821 aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat

     8881 ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga cagatccggt

     8941 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg

     9001 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta

     9061 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct

     9121 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc

     9181 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc

     9241 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat

     9301 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc

     9361 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc

     9421 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc

     9481 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat

     9541 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga

     9601 tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc gggcagttcg

     9661 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg

     9721 ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag

     9781 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca

     9841 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc

     9901 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg

     9961 agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg agagagatag

    10021 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta

    10081 tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag

    10141 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg

    10201 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata

    10261 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt

    10321 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt

    10381 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag

    10441 tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg

    10501 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca

    10561 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag

    10621 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat

    10681 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct

    10741 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttggcc

    10801 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag

    10861 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca

    10921 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag

    10981 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttggctgcag

    11041 gtcgacggat ccccgggaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc

    11101 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata

    11161 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaag cctagggagg

    11221 agtccac

pCambia1391Z其他植物表达载体：